Amnion Associated Transmembrane Protein Recombinant Protein | AMN recombinant protein

Recombinant Human Amnion Associated Transmembrane Protein

Cat. Num. AAA146013

• Gene Names: AMN; PRO1028; amnionless
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Amnion Associated Transmembrane Protein; Recombinant Human Amnion Associated Transmembrane Protein; AMN Human; Amnion Associated Transmembrane Protein Human Recombinant; Protein amnionless; AMN; PRO1028; amnionless; AMN recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: AMN protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSVSKLWVP NTDFDVAANW SQNRTPCAGG AVEFPADKMV SVLVQEGHAV SDMLLPLDGE LVLASGAGFG VSDVGSHLDC GAGEPAVFRD SDRFSWHDPH LWRSGDEAPG LFFVDAERVP CRHDDVFFPP SASFRVGLGP GASPVRVRSI SALGRTFTRD EDLAVFLASR AGRLRFHGPG ALSVGPEDCA DPSGCVCGNA EAQPWICAAL LQPLGGRCPQ AACHSALRPQ GQCCDLCGAV VLLTHGPAFD LERYRARILD TFLGLPQYHG LQVAVSKVPR SSRLREADTE IQVVLVENGP ETGGAGRLAR ALLADVAENG EALGVLEATM RESGAHVWGS S.
• Sequence Length: 453

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for AMN recombinant protein

Description: AMN Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 361 amino acids (20-357 a.a) and having a molecular mass of 38.2kDa.AMN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Amnion Associated Transmembrane Protein (AMN) is a type I transmembrane protein. The AMN protein is essential for efficient absorption of vitamin B12. AMN is assumed to modulate bone morphogenetic protein (BMP) receptor function by acting as an accessory or coreceptor thus facilitating or hindering BMP binding. A complex of AMN and cubilin forms the cubam receptor.

Product Categories/Family for AMN recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 110611172
• NCBI GeneID: 81693
• NCBI Accession #: NP_112205.2
• NCBI GenBank Nucleotide #: NM_030943.3
• UniProt Accession #: Q9BXJ7; Q6UX83
• Molecular Weight: 47,754 Da
• NCBI Official Full Name: protein amnionless
• NCBI Official Synonym Full Names: amnion associated transmembrane protein
• NCBI Official Symbol: AMN
• NCBI Official Synonym Symbols: PRO1028; amnionless
• NCBI Protein Information: protein amnionless; amnionless homolog; visceral endoderm-specific type 1 transmembrane protein
• UniProt Protein Name: Protein amnionless
• Protein Family: AMP nucleosidase
• UniProt Gene Name: AMN
• UniProt Entry Name: AMNLS_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008]

Uniprot Description

AMN: Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm. Defects in AMN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. 1 isoforms of the human protein are produced by alternative promoter.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q32.3

Cellular Component: extracellular space; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane

Molecular Function: receptor binding

Biological Process: receptor-mediated endocytosis; vitamin metabolic process; cobalamin metabolic process; multicellular organismal development; cobalamin transport; Golgi to plasma membrane protein transport; lipoprotein metabolic process; excretion; water-soluble vitamin metabolic process

Disease: Megaloblastic Anemia 1