Goat Augmenter of Liver Regeneration ELISA Kit | ALR elisa kit

Goat Augmenter of Liver Regeneration ELISA Kit

Cat. Num. AAA740650

• Gene Names: KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
• Reactivity: Goat
• Synonyms: Augmenter of Liver Regeneration; Goat Augmenter of Liver Regeneration ELISA Kit; ALR elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ALR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat ALR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALR elisa kit

Principle of the Assay: ALR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALR antibody and an ALR-HRP conjugate. The assay sample and buffer are incubated together with ALR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALR concentration since ALR from samples and ALR-HRP conjugate compete for the anti-ALR antibody binding site. Since the number of sites is limited, as more sites are occupied by ALR from the sample, fewer sites are left to bind ALR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ALR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 2358287
• NCBI GeneID: 8085
• Molecular Weight: 593,677 Da
• NCBI Official Full Name: ALR
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2D
• NCBI Official Symbol: KMT2D
• NCBI Official Synonym Symbols: ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
• NCBI Protein Information: histone-lysine N-methyltransferase 2D; ALL1-related protein; Kabuki make-up syndrome; Kabuki mental retardation syndrome; trinucleotide repeat containing 21; histone-lysine N-methyltransferase MLL2; myeloid/lymphoid or mixed-lineage leukemia 2
• UniProt Protein Name: Histone-lysine N-methyltransferase 2D
• Protein Family: Alanine racemase
• UniProt Gene Name: KMT2D
• UniProt Synonym Gene Names: ALR; MLL2; MLL4; Lysine N-methyltransferase 2D
• UniProt Entry Name: KMT2D_HUMAN

NCBI Description

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]

Uniprot Description

MLL4: Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Defects in MLL2 are the cause of Kabuki syndrome type 1 (KABUK1). A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Methyltransferase, protein lysine; Nuclear receptor co-regulator; Methyltransferase; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific)

Biological Process: oogenesis; oocyte growth; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estrogen stimulus; positive regulation of cell proliferation; chromatin silencing; positive regulation of estrogen receptor signaling pathway; histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter

Disease: Kabuki Syndrome 1