Bovine alpha2-Macroglobulin ELISA Kit | alpha2-MG elisa kit

Bovine alpha2-Macroglobulin ELISA Kit

Cat. Num. AAA738681

• Gene Names: A2M; A2MD; CPAMD5; FWP007; S863-7
• Reactivity: Bovine
• Synonyms: alpha2-Macroglobulin; Bovine alpha2-Macroglobulin ELISA Kit; Bovine a2-Macroglobulin ELISA Kit; alpha2-MG elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000ng/ml
• Sensitivity: 1.0ng/ml
• Intended Uses: This alpha2MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine alpha2MG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for alpha2-MG elisa kit

Principle of the assay: alpha2MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha2MG antibody and an alpha2MG-HRP conjugate. The assay sample and buffer are incubated together with alpha2MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha2MG concentration since alpha2MG from samples and alpha2MG-HRP conjugate compete for the anti-alpha2MG antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha2MG from the sample, fewer sites are left to bind alpha2MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha2MG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for alpha2-MG elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 825615
• NCBI GeneID: 2
• UniProt Accession #: P01023; Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97
• Molecular Weight: 163,291 Da
• NCBI Official Full Name: alpha2-macroglobulin, partial
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2M
• NCBI Official Synonym Symbols: A2MD; CPAMD5; FWP007; S863-7
• NCBI Protein Information: alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• UniProt Protein Name: Alpha-2-macroglobulin
• UniProt Gene Name: A2M
• UniProt Synonym Gene Names: CPAMD5; FWP007; Alpha-2-M
• UniProt Entry Name: A2MG_HUMAN

NCBI Description

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

Uniprot Description

A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency