Typical Testing Data/Standard Curve (for reference only)
Canine alpha Synuclein ELISA Kit | alpha-SYN elisa kit
Canine alpha Synuclein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SNCalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SNCalpha antibody and an SNCalpha-HRP conjugate. The assay sample and buffer are incubated together with SNCalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNCalpha concentration since SNCalpha from samples and SNCalpha-HRP conjugate compete for the anti-SNCalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by SNCalpha from the sample, fewer sites are left to bind SNCalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNCalpha concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.
Protein type: Adaptor/scaffold
Cellular Component: Golgi apparatus; nuclear outer membrane; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; rough endoplasmic reticulum; mitochondrion; cell; fibril; terminal button; inclusion body; cell cortex; cytosol; actin cytoskeleton; platelet alpha granule membrane; synaptic vesicle; cytoskeleton; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; synapse; nerve terminal; nucleus
Molecular Function: identical protein binding; histone binding; zinc ion binding; kinesin binding; ferrous iron binding; metal ion binding; magnesium ion binding; Hsp70 protein binding; oxidoreductase activity; calcium ion binding; enzyme binding; phospholipase binding; phospholipid binding; alpha-tubulin binding; protein domain specific binding; microtubule binding; caspase inhibitor activity; beta-tubulin binding; phosphoprotein binding; protein N-terminus binding; arachidonic acid binding; dynein binding; protein binding; copper ion binding; tau protein binding
Biological Process: regulation of acyl-CoA biosynthetic process; adult locomotory behavior; regulation of neurotransmitter secretion; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; positive regulation of neurotransmitter secretion; dopamine metabolic process; response to magnesium ion; regulation of neuron apoptosis; synapse organization and biogenesis; negative regulation of neuron apoptosis; response to drug; negative regulation of dopamine metabolic process; synaptic vesicle endocytosis; negative regulation of transporter activity; regulation of neuronal synaptic plasticity; negative regulation of apoptosis; regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; synaptic transmission, dopaminergic; negative regulation of norepinephrine uptake; mitochondrial membrane organization and biogenesis; microglial cell activation; negative regulation of monooxygenase activity; negative regulation of caspase activity; fatty acid metabolic process; regulation of dopamine secretion; positive regulation of synaptic transmission; negative regulation of dopamine uptake; negative regulation of histone acetylation; synaptic transmission; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; response to iron(II) ion; positive regulation of receptor recycling; caspase activation; neutral lipid metabolic process; protein destabilization; regulation of macrophage activation; negative regulation of microtubule polymerization; regulation of glutamate secretion; synaptic vesicle transport; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; regulation of locomotion; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential
Research Articles on alpha-SYN
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Product Notes
The Canine alpha-SYN snca (Catalog #AAA739584) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739584 ELISA Kit recognizes Canine alpha-SYN. It is sometimes possible for the material contained within the vial of "alpha Synuclein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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