Sheep Alpha-2-macroglobulin receptor-associated protein ELISA Kit | Alpha-2-MRAP elisa kit

Sheep Alpha-2-macroglobulin receptor-associated protein ELISA Kit

Cat. Num. AAA751410

• Gene Names: LRPAP1; RAP; MRAP; A2RAP; HBP44; MYP23; A2MRAP; alpha-2-MRAP
• Reactivity: Sheep
• Synonyms: Alpha-2-macroglobulin receptor-associated protein; Sheep Alpha-2-macroglobulin receptor-associated protein ELISA Kit; Alpha-2-MRAP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This LRPAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep LRPAP1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This LRPAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep LRPAP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Alpha-2-MRAP elisa kit

Principle of the Assay: LRPAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LRPAP1 antibody and an LRPAP1-HRP conjugate. The assay sample and buffer are incubated together with LRPAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRPAP1 concentration since LRPAP1 from samples and LRPAP1-HRP conjugate compete for the anti-LRPAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRPAP1 from the sample, fewer sites are left to bind LRPAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRPAP1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505021
• NCBI GeneID: 4043
• NCBI Accession #: NP_002328.1
• NCBI GenBank Nucleotide #: NM_002337.3
• Molecular Weight: 41,466 Da
• NCBI Official Full Name: alpha-2-macroglobulin receptor-associated protein
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein associated protein 1
• NCBI Official Symbol: LRPAP1
• NCBI Official Synonym Symbols: RAP; MRAP; A2RAP; HBP44; MYP23; A2MRAP; alpha-2-MRAP
• NCBI Protein Information: alpha-2-macroglobulin receptor-associated protein; 39 kDa receptor-associated protein; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1)
• UniProt Protein Name: Alpha-2-macroglobulin receptor-associated protein
• Protein Family: Alpha-2-macroglobulin receptor-associated protein
• UniProt Gene Name: LRPAP1
• UniProt Synonym Gene Names: A2MRAP; Alpha-2-MRAP; RAP
• UniProt Entry Name: AMRP_HUMAN

NCBI Description

This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cell surface; endoplasmic reticulum; integral to membrane; extracellular region; plasma membrane; vesicle

Molecular Function: heparin binding; receptor antagonist activity; protein binding; low-density lipoprotein receptor binding; unfolded protein binding; asialoglycoprotein receptor activity

Biological Process: vesicle-mediated transport; receptor-mediated endocytosis; protein folding; negative regulation of protein binding

Disease: Myopia 23, Autosomal Recessive