Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) Recombinant Protein | ALDH7A1 recombinant protein

Recombinant Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)

Cat. Num. AAA2011373

• Gene Names: ALDH7A1; EPD; PDE; ATQ1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Aldehyde Dehydrogenase 7 Family; Member A1 (ALDH7A1); Recombinant Aldehyde Dehydrogenase 7 Family; ALDH7A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-MWRLPRALCV HAAKTSKLSG PWSRPAAFMS TLLINQPQYA WLKELGLREE NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASVADYEE TVKKAREAWK IWADIPAPKR GEIVRQIGDA LREKIQVLGS LVSLEMGKIL VEGVGEVQEY VDICDYAVGL SRMIGGPILP SERSGHALIE QWNPVGLVGI ITAFNFPVAV YGWNNAIAMI CGNVCLWKGA PTTSLISVAV TKIIAKVLED NKLPGAICSL TCGGADIGTA MAKDERVNLL SFTGSTQVGK QVGLMVQERF GRSLLELGGN NAIIAFEDAD LSLVVPSALF AAVGTAGQRC TTARRLFIHE SIHDEVVNRL KKAYAQIRVG NPWDPNVLYG PLHTKQAVSM FLGAVEEAKK EGGTVVYGGK VMDRPGNYVE PTIVTGLGHD ASIAHTETFA PILYVFKFKN EEEVFAWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSKDL PLAQGIKFQ
• Sequence Length: 539

• Applicable Applications for ALDH7A1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln539 (Accession # P49419) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 188035924
• NCBI GeneID: 501
• NCBI Accession #: NP_001173.2
• NCBI GenBank Nucleotide #: NM_001182.4
• UniProt Accession #: P49419; O14619; Q6IPU8; Q9BUL4; B2R669; B4DIC7; B4DMA0; E7EPT3
• Molecular Weight: 59.7kDa
• NCBI Official Full Name: alpha-aminoadipic semialdehyde dehydrogenase isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 7 family, member A1
• NCBI Official Symbol: ALDH7A1
• NCBI Official Synonym Symbols: EPD; PDE; ATQ1
• NCBI Protein Information: alpha-aminoadipic semialdehyde dehydrogenase; antiquitin-1; P6c dehydrogenase; alpha-AASA dehydrogenase; 26g turgor protein homolog; betaine aldehyde dehydrogenase; delta1-piperideine-6-carboxylate dehydrogenase
• UniProt Protein Name: Alpha-aminoadipic semialdehyde dehydrogenase
• Protein Family: Alpha-aminoadipic semialdehyde dehydrogenase
• UniProt Gene Name: ALDH7A1
• UniProt Synonym Gene Names: ATQ1; Alpha-AASA dehydrogenase; P6c dehydrogenase
• UniProt Entry Name: AL7A1_HUMAN

NCBI Description

The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified. [provided by RefSeq, Jan 2011]

Uniprot Description

ALDH7A1: Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism. Defects in ALDH7A1 are the cause of pyridoxine-dependent epilepsy (PDE). PDE is characterized by a combination of various seizure types. It usually occurs in the first hours of life and is unresponsive to standard anticonvulsants, responding only to immediate administration of pyridoxine hydrochloride. Belongs to the aldehyde dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.31; Carbohydrate Metabolism - ascorbate and aldarate; EC 1.2.1.8; EC 1.2.1.3; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - lysine degradation; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - tryptophan; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - butanoate

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: L-aminoadipate-semialdehyde dehydrogenase activity; protein binding; aldehyde dehydrogenase (NAD) activity; betaine-aldehyde dehydrogenase activity

Biological Process: sensory perception of sound; glycine betaine biosynthetic process from choline; lysine catabolic process; aldehyde metabolic process

Disease: Epilepsy, Pyridoxine-dependent