SDS-Page
AKAP7 recombinant protein
AKAP7, 1-81aa, Human, His tag, E Coli
Gene Names
AKAP7; AKAP15; AKAP18
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
AKAP7; 1-81aa; Human; His tag; E Coli; A-kinase anchor protein 7 isoform gamma isoform alpha; AKAP15; AKAP18; AKAP7 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGQLCC FPFSRDEGKI SEKNGGEPDD AELVRLSKRL VENAVLKAVQ QYLEETQNKN KPGEGSSVKT EAADQNGNDN ENNRK
Sequence Length
81
Applicable Applications for AKAP7 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for AKAP7 recombinant protein
AKAP7 (A-kinase anchor protein 7 isoform gamma isoform alpha), also known as AKAP18, belongs to the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAP 7 is expressed in brain, heart, lung, pancreas and skeletal muscle. AKAP 7 binds PKA to the plasma membrane, and permits functional coupling to the L-type calcium channel. Recombinant human AKAP7 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for AKAP7 recombinant protein
References
Trotter K.W., et al. (1999) J. Cell Biol. 147:1481-1492; Klussmann, E., et al. (2001) Kidney Int. 60: 446-449.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11.5kDa (105aa), confirmed by MALDI-TOF
NCBI Official Full Name
A-kinase anchoring protein 7 isoform alpha
NCBI Official Synonym Full Names
A-kinase anchoring protein 7
NCBI Official Symbol
AKAP7
NCBI Official Synonym Symbols
AKAP15; AKAP18
NCBI Protein Information
A-kinase anchor protein 7; A-kinase anchoring protein 7
UniProt Protein Name
A-kinase anchor protein 7 isoforms alpha and beta
Protein Family
UniProt Gene Name
AKAP7
UniProt Synonym Gene Names
AKAP15; AKAP18; AKAP-7 isoforms alpha and beta; AKAP 18; PRKA7 isoforms alpha/beta
NCBI Description
This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Apr 2011]
Uniprot Description
Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium.
Research Articles on AKAP7
1. Malonate in the nucleotide-binding site traps human AKAP18-gamma in a novel conformational state.
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Product Notes
The AKAP7 akap7 (Catalog #AAA204701) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's AKAP7 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the AKAP7 akap7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMGQLCC FPFSRDEGKI SEKNGGEPDD AELVRLSKRL VENAVLKAVQ QYLEETQNKN KPGEGSSVKT EAADQNGNDN ENNRK. It is sometimes possible for the material contained within the vial of "AKAP7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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