AGRN / Agrin Recombinant Protein | AGRN recombinant protein

Human AGRN / Agrin Recombinant Protein

• Gene Names: AGRN; CMS8; AGRIN; CMSPPD
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: AGRN / Agrin; Human AGRN / Agrin Recombinant Protein; Agrin; AGRIN; AGRN recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 2026

• Applicable Applications for AGRN recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Monomer (By similarity). Interacts (N-terminal subunit) with TGF-beta family members, BMP2 AND BMP4; the interactions inhibit the activity of these growth factors. Interacts with TGFB1; the interaction enhances the activity of TGFB1 (By similarity). Component of the AGRN-LRP4 complex that consists of a tetramer of two AGRN-LRP4 heterodimers. Interacts (via the laminin G-like 3 domain) directly with LRP4; the interaction is required for activation of MUSK and clustering of AChR and requires the 'z8' insert present in the z(+8) isoforms. Interacts with DAG1; the interaction is influenced by cell surface glycosaminoglycans and by alternative splicing of AGRN.
• Entry Function: Agrin C-terminal 22 kDa fragment: this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 2988422
• NCBI GeneID: 375790
• UniProt Accession #: O00468
• Molecular Weight: 16.39
• NCBI Official Full Name: agrin, partial
• NCBI Official Synonym Full Names: agrin
• NCBI Official Symbol: AGRN
• NCBI Official Synonym Symbols: CMS8; AGRIN; CMSPPD
• NCBI Protein Information: agrin
• UniProt Protein Name: Agrin
• Protein Family: Agrin
• UniProt Gene Name: AGRN
• UniProt Synonym Gene Names: AGRIN; C90; C22
• UniProt Entry Name: AGRIN_HUMAN

NCBI Description

This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.

Protein type: Secreted; Cell adhesion; Extracellular matrix; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; cell surface; Golgi lumen; cytoplasm; integral to membrane; extracellular region; plasma membrane; synapse; basal lamina; cell junction

Molecular Function: heparan sulfate proteoglycan binding; protein binding; structural constituent of cytoskeleton; acetylcholine receptor regulator activity; laminin binding; calcium ion binding; sialic acid binding

Biological Process: positive regulation of filopodium formation; phototransduction, visible light; axon guidance; clustering of voltage-gated sodium channels; extracellular matrix organization and biogenesis; acetylcholine receptor signaling, muscarinic pathway; glycosaminoglycan metabolic process; neurotransmitter receptor metabolic process; pathogenesis; positive regulation of synaptic growth at neuromuscular junction; signal transduction; receptor clustering; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; plasma membrane organization and biogenesis; positive regulation of neuron apoptosis; carbohydrate metabolic process; synapse organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; retinoid metabolic process; neuromuscular junction development

Disease: Myasthenic Syndrome, Congenital, With Pre- And Postsynaptic Defects