Argonaute-2 Recombinant Protein | AGO2 recombinant protein

Recombinant Human Protein argonaute-2

Cat. Num. AAA1430889

• Gene Names: AGO2; Q10; EIF2C2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Argonaute-2; Recombinant Human Protein argonaute-2; Argonaute RISC catalytic component 2; AGO2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 517-818aa; Partial
• Sequence: LVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLV

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for AGO2 recombinant protein

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complentary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complentarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complentary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complentary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complentary promoter regions

Product Categories/Family for AGO2 recombinant protein

Epigenetics and Nuclear Signaling

References

DNA sequence and analysis of human chromosome 8.Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.Nature 439:331-335(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 257467482
• NCBI GeneID: 27161
• NCBI Accession #: NP_001158095.1
• NCBI GenBank Nucleotide #: NM_001164623.1
• UniProt Accession #: Q9UKV8; Q8TCZ5; Q8WV58; Q96ID1
• Molecular Weight: 36.1 kDa
• NCBI Official Full Name: protein argonaute-2 isoform 2
• NCBI Official Synonym Full Names: argonaute 2, RISC catalytic component
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: Q10; EIF2C2
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: EIF2C2; Argonaute2; hAgo2; eIF-2C 2; eIF2C 2; PPD
• UniProt Entry Name: AGO2_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA-binding; EC 3.1.26.n2; RNA processing

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: cytoplasm; cytosol; dendrite; membrane; mRNA cap complex; nucleoplasm; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endoribonuclease activity; metal ion binding; miRNA binding; mRNA binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; single-stranded RNA binding; siRNA binding; translation initiation factor activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; gene expression; innate immune response; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; negative regulation of translational initiation; nerve growth factor receptor signaling pathway; Notch signaling pathway; phosphoinositide-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; pre-microRNA processing; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; transcription, DNA-dependent; translation; translational initiation