AGK recombinant protein

Recombinant Human AGK protein

Cat. Num. AAA1562781

• Gene Names: AGK; MULK; CATC5; CTRCT38; MTDPS10
• Purity: >90% as determined by SDS-PAGE
• Synonyms: AGK; Recombinant Human AGK protein; Acylglycerol kinase; mitochondrial; hAGK; Multiple substrate lipid kinase; HsMuLK; MuLK; Multi-substrate lipid kinase; AGK recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human AGK (Thr15-Pro300) was fused with GST tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for AGK recombinant protein

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Phosphorylates ceramide. Phosphorylates 1, 2-dioleoylglycerol more rapidly than 2, 3-dioleoylglycerol. Independently of its lipid kinase activity, acts as a component of the TIM22 complex. The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.

Product Categories/Family for AGK recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 8922701
• NCBI GeneID: 55750
• NCBI Accession #: NP_060708.1
• NCBI GenBank Nucleotide #: NM_018238.3
• UniProt Accession #: Q53H12; Q75KN1; Q96GC3; Q9NP48
• Molecular Weight: 47,137 Da
• NCBI Official Full Name: acylglycerol kinase, mitochondrial
• NCBI Official Synonym Full Names: acylglycerol kinase
• NCBI Official Symbol: AGK
• NCBI Official Synonym Symbols: MULK; CATC5; CTRCT38; MTDPS10
• NCBI Protein Information: acylglycerol kinase, mitochondrial; hAGK; hsMuLK; multi-substrate lipid kinase; multiple substrate lipid kinase
• UniProt Protein Name: Acylglycerol kinase, mitochondrial
• Protein Family: Acylglycerol kinase
• UniProt Gene Name: AGK
• UniProt Synonym Gene Names: MULK; hAGK; HsMuLK; MuLK
• UniProt Entry Name: AGK_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]

Uniprot Description

AGK: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. Defects in AGK are the cause of mitochondrial DNA depletion syndrome type 10 (MTDPS10). An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy. Defects in AGK are the cause of cataract, congenital, autosomal recessive type 5 (CATC5). CATC5 consists of an opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Kinase, lipid; EC 2.7.1.107; EC 2.7.1.94

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: mitochondrion; intracellular membrane-bound organelle; mitochondrial membrane

Molecular Function: acylglycerol kinase activity; ceramide kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein kinase C activation; ceramide biosynthetic process; glycerolipid metabolic process; lipid phosphorylation

Disease: Cataract 38; Sengers Syndrome