Mouse adiponectin, C1Q and collagen domain containing ELISA Kit | ADP elisa kit

Mouse adiponectin, ADP ELISA Kit

Cat. Num. AAA704558

• Gene Names: Adipoq; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
• Reactivity: Mouse
• Synonyms: adiponectin; C1Q and collagen domain containing; Mouse adiponectin; ADP ELISA Kit; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; OTTHUMP00000210047; OTTHUMP00000210048; adipocyte; C1Q and collagen domain-containing; adipo; ADP; ADP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ADIPOQ. No significant cross-reactivity or interference between Mouse ADIPOQ and analogues was observed.
• Sequence Length: 247

• Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cardiovascular
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADP elisa kit

Introduction: Adiponectin (also referred to as Acrp30, apM1) is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid catabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones. Levels of the hormone are inversely correlated with body fat percentage in adults, while the association in infants and young children is more unclear. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin is a 244-amino-acid-long polypeptide. There are four distinct regions of adiponectin. The first is a short signal squence that targets the hormone for secretion outside the cell; next is a short region that varies between species; the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors. However, when the 3-dimensional structure of the globular region was determined, a striking similarity to TNFalpha was observed, despite unrelated protein sequences. Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein at around 5-10 ug/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ADP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 87252711
• NCBI GeneID: 11450
• NCBI Accession #: NP_033735.3
• NCBI GenBank Nucleotide #: NM_009605.4
• UniProt Accession #: Q60994; Q62400; Q6GTX4; Q9DC68
• Molecular Weight: 26,809 Da
• NCBI Official Full Name: adiponectin
• NCBI Official Synonym Full Names: adiponectin, C1Q and collagen domain containing
• NCBI Official Symbol: Adipoq
• NCBI Official Synonym Symbols: APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
• NCBI Protein Information: adiponectin; adipocyte-specific protein AdipoQ; adipocyte complement related protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein
• UniProt Protein Name: Adiponectin
• UniProt Gene Name: Adipoq
• UniProt Synonym Gene Names: Acdc; Acrp30; Apm1; ACRP30
• UniProt Entry Name: ADIPO_MOUSE

Uniprot Description

Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.5 PublicationsManual assertion based on experiment in:Ref.11