Human Adiponectin receptor protein 1 ELISA Kit | ADIPOR1 elisa kit

Human Adiponectin receptor protein 1 ELISA Kit

Cat. Num. AAA762630

• Gene Names: ADIPOR1; CGI45; PAQR1; ACDCR1; CGI-45; TESBP1A
• Reactivity: Human
• Synonyms: Adiponectin receptor protein 1; Human Adiponectin receptor protein 1 ELISA Kit; ADIPOR1/ACDCR1/CGI45/PAQR1/TESBP1A/ACDCR1/adiponectin receptor 1/adiponectin receptor protein 1/ADR1/CGI45/FLJ42464/PAQR1FLJ25385/Progestin and adipoQ receptor family member I/TESBP1A; ADIPOR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADIPOR1. No significant cross-reactivity or interference between ADIPOR1 and analogues was observed.
• Sequence Length: 375

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADIPOR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 594952032
• NCBI GeneID: 51094
• NCBI Accession #: NP_001277558.1
• NCBI GenBank Nucleotide #: NM_001290629.1
• Molecular Weight: 42,616 Da
• NCBI Official Full Name: adiponectin receptor protein 1
• NCBI Official Synonym Full Names: adiponectin receptor 1
• NCBI Official Symbol: ADIPOR1
• NCBI Official Synonym Symbols: CGI45; PAQR1; ACDCR1; CGI-45; TESBP1A
• NCBI Protein Information: adiponectin receptor protein 1
• UniProt Protein Name: Adiponectin receptor protein 1
• Protein Family: Adiponectin receptor protein
• UniProt Gene Name: ADIPOR1
• UniProt Entry Name: ADR1_HUMAN

NCBI Description

This gene encodes a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signaling pathway which affects levels of fatty acid oxidation and insulin sensitivity. A pseudogene of this gene is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

ADIPOR1: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin. Belongs to the ADIPOR family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: integral to membrane; intrinsic to plasma membrane; membrane; plasma membrane

Molecular Function: adiponectin binding; identical protein binding; metal ion binding; protein heterodimerization activity; protein kinase binding

Biological Process: adiponectin-mediated signaling pathway; fatty acid oxidation; G-protein coupled receptor protein signaling pathway; glucose homeostasis; hormone-mediated signaling; leptin-mediated signaling pathway; negative regulation of cell growth; positive regulation of insulin receptor signaling pathway; positive regulation of JAK-STAT cascade; regulation of lipid metabolic process