mAdiponectin Recombinant Protein | Adipoq recombinant protein

mAdiponectin, 18- 247aa, Mouse, His tag, E Coli

Cat. Num. AAA203136

• Gene Names: Adipoq; Ad; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: mAdiponectin; 18- 247aa; Mouse; His tag; E Coli; adipocyte complement-related protein; Acdc; GBP28; Acrp30; His-tagged; Adiponectin; C1Q and collagen domain containing [Mus musculus]; ; Adipoq recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: 20mM Tris pH 8.0, 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MEDDVTTTEE LAPALVPPPK GTCAGWMAGI PGHPGHNGTP GRDGRDGTPG EKGEKGDAGL LGPKGETGDV GMTGAEGPRG FPGTPGRKGE PGEAAYVYRS AFSVGLETRV TVPNVPIRFT KIFYNQQNHY DGSTGKFYCN IPGLYYFSYH ITVYMKDVKV SLFKKDKAVL FTYDQYQEKN VDQASGSVLL HLEVGDQVWL QVYGDGDHNG LYADNVNDST FTGFLLYHDT N
• Sequence Length: 247

• Applicable Applications for Adipoq recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Adipoq recombinant protein

Adiponectin is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Adiponectin is reduced in obese humans, and decreased level is associated with insulin resistance and hyperinsulinemia. Recombinant mouse adiponectin, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for Adipoq recombinant protein

Hormones & Cytokines

References

Yokota. T. et al. (2000) Blood. 96:1723; Yamauchi T. et al (2002) Nature Medicine.8 (11):1288-1295; Lu M. et al (2007) Mol Endocrinol. 59 (2):207-16;

NCBI and Uniprot Product Information

• NCBI GI #: 87252711
• NCBI GeneID: 11450
• NCBI Accession #: NP_033735
• NCBI GenBank Nucleotide #: NM_009605.5
• UniProt Accession #: Q60994; Q62400; Q6GTX4; Q9DC68
• Molecular Weight: 27.2 kDa (251 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: adiponectin
• NCBI Official Synonym Full Names: adiponectin, C1Q and collagen domain containing
• NCBI Official Symbol: Adipoq
• NCBI Official Synonym Symbols: Ad; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
• NCBI Protein Information: adiponectin
• UniProt Protein Name: Adiponectin
• Protein Family: Adiponectin
• UniProt Gene Name: Adipoq
• UniProt Synonym Gene Names: Acdc; Acrp30; Apm1; ACRP30

Uniprot Description

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.