Mouse A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4 ELISA Kit | ADAMTS4 elisa kit

Mouse A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4 ELISA Kit

Cat. Num. AAA733340

• Gene Names: ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4
• Reactivity: Mouse
• Synonyms: A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4; Mouse A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4 ELISA Kit; ADAMTS4 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ADAMTS4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ADAMTS4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS4 elisa kit

Principle of the assay: ADAMTS4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMTS4 antibody and an ADAMTS4-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS4 concentration since ADAMTS4 from samples and ADAMTS4-HRP conjugate compete for the anti-ADAMTS4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS4 from the sample, fewer sites are left to bind ADAMTS4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAMTS4 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 157427675
• NCBI GeneID: 9507
• NCBI Accession #: NP_005090.3
• NCBI GenBank Nucleotide #: NM_005099.4
• Molecular Weight: 90,197 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• NCBI Official Symbol: ADAMTS4
• NCBI Official Synonym Symbols: ADMP-1; ADAMTS-2; ADAMTS-4
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 4
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS4
• UniProt Synonym Gene Names: KIAA0688; UNQ769/PRO1563; ADAM-TS 4; ADAM-TS4; ADAMTS-4
• UniProt Entry Name: ATS4_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Protein type: EC 3.4.24.82; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; protein binding; protease binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development