SDS-Page
A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12) Recombinant Protein | ADAMTS12 recombinant protein
Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)
Gene Names
Adamts12; AI605170; ADAMTS-12
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12); Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12); ADAMTS12 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-SPA YWIVGNWSKC STTCGLGAYW RSVECSSGVD ADCTTIQRPD PAKKCHLRPC AGWRVGNWSK CSRNCSGGFK IREVQCMDSL DHHRSLRPFH CQFLAGAPPP LSMSCNLEPC GEWQVEPWSQ CSRSCGGGVQ ERGVSCPGGL CDWTKRPATT VPCNRHLCCH WATGNWELCN TSCGGGSQKR TIHCIPSENS TTEDQDQCLC DHQVKPPEFQ TCNQQACR
MGHHHHHHSGS-SPA YWIVGNWSKC STTCGLGAYW RSVECSSGVD ADCTTIQRPD PAKKCHLRPC AGWRVGNWSK CSRNCSGGFK IREVQCMDSL DHHRSLRPFH CQFLAGAPPP LSMSCNLEPC GEWQVEPWSQ CSRSCGGGVQ ERGVSCPGGL CDWTKRPATT VPCNRHLCCH WATGNWELCN TSCGGGSQKR TIHCIPSENS TTEDQDQCLC DHQVKPPEFQ TCNQQACR
Sequence Length
1600
Applicable Applications for ADAMTS12 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ser1318-Arg1538 (Accession # Q811B3) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.7kDa
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 12
NCBI Official Synonym Full Names
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12
NCBI Official Symbol
Adamts12
NCBI Official Synonym Symbols
AI605170; ADAMTS-12
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM-TS12; ADAM-TS 12; metalloprotease disintegrin 12; a disintegrin-like and metalloprotease with thrombospondin type 1 motif, 12; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 12
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 12
UniProt Gene Name
Adamts12
UniProt Synonym Gene Names
ADAM-TS 12; ADAM-TS12; ADAMTS-12
UniProt Entry Name
ATS12_MOUSE
Uniprot Description
ADAMTS12: Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; EC 3.4.24.-; Protease; Secreted; Secreted, signal peptide
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region
Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metal ion binding; metalloendopeptidase activity
Biological Process: cell migration; proteolysis involved in cellular protein catabolic process; cell-matrix adhesion; negative regulation of chondrocyte differentiation; regulation of inflammatory response; proteoglycan catabolic process; proteolysis
Research Articles on ADAMTS12
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Product Notes
The ADAMTS12 adamts12 (Catalog #AAA2011347) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the ADAMTS12 adamts12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG S-SPA YWIVGNWSKC STTCGLGAYW RSVECSSGVD ADCTTIQRPD PAKKCHLRPC AGWRVGNWSK CSRNCSGGFK IREVQCMDSL DHHRSLRPFH CQFLAGAPPP LSMSCNLEPC GEWQVEPWSQ CSRSCGGGVQ ERGVSCPGGL CDWTKRPATT VPCNRHLCCH WATGNWELCN TSCGGGSQKR TIHCIPSENS TTEDQDQCLC DHQVKPPEFQ TCNQQACR. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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