Cat A Disintegrin and Metalloprotease 10 ELISA Kit | ADAM10 elisa kit

Cat A Disintegrin and Metalloprotease 10 ELISA Kit

• Gene Names: ADAM10; kuz; AD10; MADM; CD156c; HsT18717
• Reactivity: Cat
• Synonyms: A Disintegrin and Metalloprotease 10; Cat A Disintegrin and Metalloprotease 10 ELISA Kit; ADAM10 elisa kit

• Reactivity: Cat
• Specificity: No significant cross-reactivity or interference between Cat ADAM10 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-500pg/ml.
• Sensitivity: 2.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ADAM10 concentrations in Cat serum, plasma and other body fluids. Using Purified Cat ADAM10 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ADAM10 and ADAM10 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADAM10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ADAM10 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ADAM10 concentrations in Cat serum, plasma and other body fluids. Using Purified Cat ADAM10 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ADAM10 and ADAM10 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADAM10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2393947
• NCBI GeneID: 102
• UniProt Accession #: O14672; Q10742; Q92650
• Molecular Weight: 84,142 Da
• NCBI Official Full Name: ADAM10
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: ADAM10
• NCBI Official Synonym Symbols: kuz; AD10; MADM; CD156c; HsT18717
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM10
• UniProt Synonym Gene Names: KUZ; MADM; ADAM 10
• UniProt Entry Name: ADA10_HUMAN

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Motility/polarity/chemotaxis; Protease; Cell surface; Cell adhesion; Membrane protein, integral; EC 3.4.24.81; Vesicle

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; focal adhesion; cell surface; membrane; intracellular membrane-bound organelle; postsynaptic density; cytoplasm; plasma membrane; integral to membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; SH3 domain binding; protein kinase binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; axon guidance; Notch signaling pathway; extracellular matrix organization and biogenesis; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; positive regulation of cell growth; protein amino acid phosphorylation; extracellular matrix disassembly; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; cell-cell signaling; monocyte activation; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18