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SDS-Page

Acy1 recombinant protein

Acy1, 1-408aa, Mouse, His tag, E Coli

Gene Names
Acy1; Acy-1; 1110014J22Rik
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Acy1; 1-408aa; Mouse; His tag; E Coli; Aminoacylase 1; ACY-1; ACY1_HUMAN; ACY1D; ACYLASE; Acylase I; Aminoacylase-1; EC 3.5.1.14; epididymis secretory protein Li 5; HEL-S-5; N acyl L amino acid amidohydrolase; N-acyl-L-amino-acid amidohydrolase; OTTHUMP00000212459; OTTHUMP00000212462; OTTHUMP00000212463; OTTHUMP00000212464; OTTHUMP00000212465; Acy1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid, In Phosphate buffered saline (pH 7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTTKD PESEHPSVTL FRQYLRICTV QPNPDYGGAI TFLEERARQL GLSCQKIEVV PGFVITVLTW PGTNPSLPSI LLNSHTDVVP VFKEHWHHDP FEAFKDSEGY IYARGSQDMK SVSIQYLEAV RRLKSEGHRF PRTIHMTFVP DEEVGGHKGM ELFVKRPEFQ ALRAGFALDE GLANPTDAFT VFYSERSPWW VRVTSTGKPG HASRFIEDTA AEKLHKVISS ILAFREKERQ RLQANPHLKE GAVTSVNLTK LEGGVAYNVV PATMSASFDF RVAPDVDMKA FEKQLQRWCQ EAGEGVTFEF AQKFTEPRMT PTDDSDPWWA AFSGACKAMN LTLEPEIFPA ATDSRYIRAV GIPALGFSPM NRTPVLLHDH NERLHEDIFL RGVDIYTGLL SALASVPTLP GES
Sequence Length
408
Applicable Applications for Acy1 recombinant protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for Acy1 recombinant protein
Acy1 also known as Aminoacylase1. The protein is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. Recombinant mouse Acy1, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Acy1 recombinant protein
References
Miller YE, Drabkin H, et al. (1991) Genomics 8 (1): 149-154. ; Voss R, et al. (1982) Ann Hum Genet 44 (Pt 1): 1-9.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
48.4kDa (433aa), confirmed by MALDI-TOF
NCBI Official Full Name
Aminoacylase 1
NCBI Official Synonym Full Names
aminoacylase 1
NCBI Official Symbol
Acy1
NCBI Official Synonym Symbols
Acy-1; 1110014J22Rik
NCBI Protein Information
aminoacylase-1
UniProt Protein Name
Aminoacylase-1
Protein Family
UniProt Gene Name
Acy1
UniProt Synonym Gene Names
ACY-1

Uniprot Description

Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).

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Product Notes

The Acy1 acy1 (Catalog #AAA205976) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Acy1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Acy1 acy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSEFMTTKD PESEHPSVTL FRQYLRICTV QPNPDYGGAI TFLEERARQL GLSCQKIEVV PGFVITVLTW PGTNPSLPSI LLNSHTDVVP VFKEHWHHDP FEAFKDSEGY IYARGSQDMK SVSIQYLEAV RRLKSEGHRF PRTIHMTFVP DEEVGGHKGM ELFVKRPEFQ ALRAGFALDE GLANPTDAFT VFYSERSPWW VRVTSTGKPG HASRFIEDTA AEKLHKVISS ILAFREKERQ RLQANPHLKE GAVTSVNLTK LEGGVAYNVV PATMSASFDF RVAPDVDMKA FEKQLQRWCQ EAGEGVTFEF AQKFTEPRMT PTDDSDPWWA AFSGACKAMN LTLEPEIFPA ATDSRYIRAV GIPALGFSPM NRTPVLLHDH NERLHEDIFL RGVDIYTGLL SALASVPTLP GES. It is sometimes possible for the material contained within the vial of "Acy1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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