Mouse Activin receptor type-2A ELISA Kit | Acvr2a elisa kit

Mouse Activin receptor type-2A ELISA Kit

Cat. Num. AAA765187

• Gene Names: ACVR2A; ACVR2; ACTRII
• Reactivity: Mouse
• Synonyms: Activin receptor type-2A; Mouse Activin receptor type-2A ELISA Kit; Acvr2a/Acvr2/Activin receptor type IIA/ACTR-IIA; Acvr2a elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Acvr2a. No significant cross-reactivity or interference between Acvr2a and analogues was observed.
• Sequence Length: 513

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Acvr2a elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 518828583
• NCBI GeneID: 92
• NCBI Accession #: NP_001265508.1
• NCBI GenBank Nucleotide #: NM_001278579.1
• Molecular Weight: 45,713 Da
• NCBI Official Full Name: activin receptor type-2A isoform 1
• NCBI Official Synonym Full Names: activin A receptor type IIA
• NCBI Official Symbol: ACVR2A
• NCBI Official Synonym Symbols: ACVR2; ACTRII
• NCBI Protein Information: activin receptor type-2A
• UniProt Protein Name: Activin receptor type-2A
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR2A
• UniProt Synonym Gene Names: ACVR2; ACTR-IIA; ACTRIIA
• UniProt Entry Name: AVR2A_HUMAN

NCBI Description

This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Motility/polarity/chemotaxis; Kinase, protein; EC 2.7.11.30; Protein kinase, TKL; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: cell surface; cytoplasm; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: activin binding; activin receptor activity; ATP binding; coreceptor activity; growth factor binding; metal ion binding; PDZ domain binding; protein binding; protein self-association; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; transmembrane receptor protein serine/threonine kinase activity

Biological Process: activin receptor signaling pathway; anterior/posterior pattern formation; BMP signaling pathway; determination of left/right symmetry; embryonic skeletal development; gastrulation with mouth forming second; mesoderm development; penile erection; positive regulation of activin receptor signaling pathway; positive regulation of bone mineralization; positive regulation of erythrocyte differentiation; positive regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of BMP signaling pathway; regulation of nitric-oxide synthase activity; Sertoli cell proliferation; sperm ejaculation; spermatogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway