Vascular Endothelial Growth Factor 165 Active Protein | FIGF active protein

Vascular Endothelial Growth Factor 165, Recombinant, Mouse (VEGF 165)

Cat. Num. AAA650170

• Gene Names: FIGF; VEGFD; VEGF-D
• Purity: Highly Purified; ~ 98% by SDS-PAGE and HPLC.
• Synonyms: Vascular Endothelial Growth Factor 165; Recombinant; Mouse (VEGF 165); FIGF active protein

• Purity/Purification: Highly Purified; ~ 98% by SDS-PAGE and HPLC.
• Form/Format: Supplied as a lyophilized powder with no preservatives. Reconstitute with sterile dH2O or PBS, 0.1% BSA to 0.1-1mg/ml.
• Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-5ng/ml.
• Endotoxin Level: 0.1ng/ug (1EU/ug).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile PBS or ddH2O, 0.1% BSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for FIGF active protein

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor. VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR-1 (flt-1), VEGFR-2 (flk-1/KDR), and VEGFR-3/Flt-4. Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo.

Product Categories/Family for FIGF active protein

Growth Factors; Cytokines; Growth Factors-VEGF

NCBI and Uniprot Product Information

• NCBI GI #: 4758378
• NCBI GeneID: 2277
• NCBI Accession #: NP_004460.1
• NCBI GenBank Nucleotide #: NM_004469.4
• UniProt Accession #: O43915; B2R7Z3
• Molecular Weight: 39.0kD
• NCBI Official Full Name: vascular endothelial growth factor D preproprotein
• NCBI Official Synonym Full Names: c-fos induced growth factor (vascular endothelial growth factor D)
• NCBI Official Symbol: FIGF
• NCBI Official Synonym Symbols: VEGFD; VEGF-D
• NCBI Protein Information: vascular endothelial growth factor D
• UniProt Protein Name: Vascular endothelial growth factor D
• UniProt Gene Name: FIGF
• UniProt Synonym Gene Names: VEGFD; VEGF-D; FIGF
• UniProt Entry Name: VEGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]

Uniprot Description

Function: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Ref.8

Subunit structure: Homodimer; non-covalent and antiparallel. Ref.8

Subcellular location: Secreted.

Tissue specificity: Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.

Post-translational modification: Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.