TSG-6 Active Protein | TNFAIP6 active protein

TSG-6, Recombinant, Mouse (TNF-Stimulated Gene 6, TNFIP6)

Cat. Num. AAA637947

• Gene Names: TNFAIP6; TSG6; TSG-6
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: TSG-6; Recombinant; Mouse (TNF-Stimulated Gene 6; TNFIP6); TNFAIP6 active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to bind biotinylated Hyaluronan in a functional ELISA type binding assay with an estimated KD < 1 nM.
• Endotoxin Level: < 1.0 EU per 1 g of protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for TNFAIP6 active protein

TSG-6 (TNF-stimulated gene 6; also TNFIP6) is a secreted, 35-39 kD group A member of the LINK-Module superfamily of proteins (1-4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa #36-129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa #135-246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and dog TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80-85 kD protein subunit from I I (inter- -inhibitor) to HA. I I is a four-component, 225 kD serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I I also leads to free bikunin, which in the "free" state becomes a potent inhibitor of serine proteases (8)

Product Categories/Family for TNFAIP6 active protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 26051243
• NCBI GeneID: 7130
• NCBI Accession #: NP_009046.2
• NCBI GenBank Nucleotide #: NM_007115.3
• UniProt Accession #: P98066; Q53TI7; Q8WWI9
• Molecular Weight: Based on N-terminal amino acid sequencing, the recombinant mouse TSG-6 starts at Trp 18 and has a calculated molecular mass of 29.8 kD. As a result of glycosylation, the recombinant protein migrates as an approximately 39 kD protein in SDS-PAGE under redu
• NCBI Official Full Name: tumor necrosis factor-inducible gene 6 protein
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 6
• NCBI Official Symbol: TNFAIP6
• NCBI Official Synonym Symbols: TSG6; TSG-6
• NCBI Protein Information: tumor necrosis factor-inducible gene 6 protein; TNF alpha-induced protein 6; hyaluronate-binding protein; TNF-stimulated gene 6 protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein
• UniProt Protein Name: Tumor necrosis factor-inducible gene 6 protein
• Protein Family: Tumor necrosis factor-inducible gene 6 protein
• UniProt Gene Name: TNFAIP6
• UniProt Synonym Gene Names: TSG6; TSG-6
• UniProt Entry Name: TSG6_HUMAN

NCBI Description

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]

Uniprot Description

TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: extracellular space

Molecular Function: hyaluronic acid binding

Biological Process: ovulation; cell-cell signaling; signal transduction; inflammatory response; cell adhesion; positive regulation of cell migration