Tie2, Fc Chimera, Rat Active Protein

Tie2, Fc Chimera, Recombinant, Rat (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Cat. Num. AAA637169

• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Tie2; Fc Chimera; Rat; Recombinant; Rat (Tunica Internal Endothelial Cell Kinase; Angiopoietin1 Receptor; p140 Tek; Tyrosine Protein Kinase Receptor); Rat active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS.

• Activity: Measured in a functional ELISA assay. When rrTIE-2/Fc is immobilized at 4 g/mL (100 L/well), it binds rhAngiopoietin-2 with a linear range of 0.8-50 ng/mL.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for Tie2, Fc Chimera, Rat active protein

Tie-2, also known as Tek, is a 145 kD, type I transmembrane glycoprotein receptor tyrosine kinase that is a receptor for angiopoietins. 1 The 1120 amino acid (aa) rat Tie-2 precursor contains an 18 aa signal sequence, a 723 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 354 aa cytoplasmic tail. 2 The ECD contains two C2 Ig-like domains, three EGF-like motifs, and three fibronectin type III repeats. The cytoplasmic region has a split tyrosine kinase domain and presumably autophosphorylates as a ligand-induced homodimer. 3 Rat Tie-2 ECD shares 96%, 90% and 89% aa identity with mouse, human and bovine Tie-2, respectively, and 47% aa identity with rat Tie-1 ECD. Cells known to express Tie-2 include embryonic and adult endothelial cells, hematopoietic stem cells and a circulating population of proangiogenic Tie-2 expressing monocytes (TEM). 4-7 A soluble form of Tie-2, most likely the result of proteolytic cleavage, is found in serum. 8 The four angiopoietins are ligands of Tie-2. Ang-1 and Ang-4 are Tie-2 activators, while Ang-2 and Ang-3 can be activators or inhibitors, depending on context. 1, 9 Tie-2 is said to be important for maintaining vascular integrity. It mediates endothelial cell-smooth muscle cell communication, and inhibits endothelial cell apoptosis, thus maintaining endothelial cell survival. 10-12 It is also absolutely required for embryonic development of the endocardium. 3, 10, 13 While not essential for embryonic hematopoiesis, Ang-1 production by osteoblasts promotes quiescence of Tie-2-expressing bone marrow stem cells. This quiescence is critical for maintaining an ongoing hematopoietic capability. 12, 14, 15

Product Categories/Family for Tie2, Fc Chimera, Rat active protein

Molecular Biology; MB-Receptor Tyrosine Kinases