Sialoprotein II, Bone Active Protein | IBSP active protein

Sialoprotein II, Bone, Recombinant, Mouse (Bone Sialoprotein 2, Bone Sialoprotein II, BSP II, Cell-binding Sialoprotein, Integrin-binding Sialoprotein, IBSP, BNSP)

Cat. Num. AAA636831

• Gene Names: IBSP; BSP; BNSP; SP-II; BSP-II
• Purity: Highly Purified; ~90% (SDS-PAGE, reducing conditions)
• Synonyms: Sialoprotein II; Bone; Recombinant; Mouse (Bone Sialoprotein 2; Bone Sialoprotein II; BSP II; Cell-binding Sialoprotein; Integrin-binding Sialoprotein; IBSP; BNSP); IBSP active protein

• Purity/Purification: Highly Purified; ~90% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.

• Application Notes: SDS-PAGE: 70-90kD, reducing conditions
• Activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast adenocarcinoma cells. When 5x10e4 cells/well are added to recombinant mouse IBSP coated plates (3ug/ml with 100ul/well), approximately 60-80% will adhere after 30 minutes at 37 degree C.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IBSP active protein

IBSP (integrin­binding sialoprotein; also BSP or bone sialoprotein II) is a 55­75kD, secreted, variably glycosylated, monomeric non­collagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is principally associated with the early stages of bone mineralization. Mouse BSP is synthesized as a 324aa precursor that contains a 16aa signal sequence and a 308aa mature region. The mature segment is divided into a basic N­terminus (aa17-62), a central region (aa63­233), and an acidic C­terminus (aa234­317). Functional segments associated with the mature molecule include a type I collagen binding domain (aa19­46), two non­RGD cell binding sites (aa30­57 and 261­281), an RGD alpha-v-b-3 integrin­binding site (aa286­288) and two regions that are potential hydroxyapatite (HAp) nucleation domains (aa76­83 and 151­158). HAp formation requires a BSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP­associated conucleator BSP is highly glycosylated, sulfated and phosphorylated. Phosphorylation promotes HAp nucleation, while carbohydrate may regulate cell adhesion. Interaction with integrins stimulates cell migration and survival, and has been implicated in bone metastasis of cancers, especially those of breast and prostate. Mature mouse BSP shares 90ª identity with rat, 70% with human, 67% with canine, equine and porcine, and 64% with bovine BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts and bone marrow stromal cells.

Product Categories/Family for IBSP active protein

Molecular Biology; MB-Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 338084
• NCBI GeneID: 3381
• UniProt Accession #: P21815
• Molecular Weight: ~35kD
• NCBI Official Full Name: sialoprotein
• NCBI Official Synonym Full Names: integrin-binding sialoprotein
• NCBI Official Symbol: IBSP
• NCBI Official Synonym Symbols: BSP; BNSP; SP-II; BSP-II
• NCBI Protein Information: bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein
• UniProt Protein Name: Bone sialoprotein 2
• Protein Family: Bone sialoprotein
• UniProt Gene Name: IBSP
• UniProt Synonym Gene Names: BNSP; BSP II
• UniProt Entry Name: SIAL_HUMAN

NCBI Description

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: extracellular space; membrane; extracellular region

Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion