SFRP4 active protein

SFRP4, Recombinant, Human (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2)

Cat. Num. AAA636038

• Gene Names: SFRP4; FRP-4; FRPHE; sFRP-4
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: SFRP4; Recombinant; Human (Secreted Frizzled Related Protein 4; DDC-4; FrpAP; frpHE; FrzB-2); SFRP4 active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS containing 50ug BSA per 1ug cytokine.

• Activity: Measured by its ability to inhibit Wnt-3A's ability to induce alkaline phosphatase synthesis in the; presence of BMP-2 by MC3T3E1 osteoblastic cells. The ED50 for this effect is typically 4-20ug/ml, in the presence of 20ng/ml of rmWnt-3a.
• Endotoxin Level: 1.0 EU per 1ug of the cytokine as determined by the LAL method.
• Reconstitution: Reconstitute with sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin. Prepare a stock solution of no less than 10ug/ml.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C to -70 degree C. Upon reconstitution, this cytokine can be stored under sterile conditions at 2 degree -8 degree C for one month; or at -20 degree C to -70 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for SFRP4 active protein

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. sFRPs are ~30-35kD in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with high degree of similarity to the Frizzled proteins, including 10 conserved cysteines; and a 175aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPs are thought to act as soluble antagonists of Wnt signals, often postulated to act as tumor suppressor genes (1). sFRP-4, also known as DDC-4, FrpAP, frpHE and FrzB-2, is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1,2). This protein shows complex functions with respect to cell survival: it is upregulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is upregulated in endometrial and breast carcinomas (6,7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with

Product Categories/Family for SFRP4 active protein

Molecular Biology; MB-Wnt Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 170784838
• NCBI GeneID: 6424
• NCBI Accession #: NP_003005.2
• NCBI GenBank Nucleotide #: NM_003014.3
• UniProt Accession #: Q6FHJ7; O14877; Q05BG7; Q1ZYW2; Q4G124; Q6FHM0; Q6PD64; B4DYC1
• Molecular Weight: Mature recombinant human sFRP-4 is a monomeric protein. Based on N-terminal sequencing, it starts with Ala22 at its N-terminus. As a result of glycosylation, the recombinant protein migrates; with an apparent molecular mass of ~55kD in SDS-PAGE under re
• NCBI Official Full Name: secreted frizzled-related protein 4
• NCBI Official Synonym Full Names: secreted frizzled-related protein 4
• NCBI Official Symbol: SFRP4
• NCBI Official Synonym Symbols: FRP-4; FRPHE; sFRP-4
• NCBI Protein Information: secreted frizzled-related protein 4; frizzled protein, human endometrium; secreted frizzled-related protein 4; secreted frizzled-related protein 4
• UniProt Protein Name: Secreted frizzled-related protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP4
• UniProt Synonym Gene Names: FRPHE; sFRP-4; FrpHE
• UniProt Entry Name: SFRP4_HUMAN

NCBI Description

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Uniprot Description

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding

Biological Process: negative regulation of cell proliferation; positive regulation of apoptosis; multicellular organismal development; negative regulation of transcription factor activity; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; response to hormone stimulus; phosphate ion homeostasis; cell differentiation