Renin Active Protein | REN active protein

Renin, Recombinant, Mouse

Cat. Num. AAA635990

• Gene Names: REN; HNFJ2
• Purity: Purified; 95% (SDS­PAGE)
• Synonyms: Renin; Recombinant; Mouse; REN active protein

• Purity/Purification: Purified; 95% (SDS­PAGE)
• Form/Format: Supplied as a liquid in MES, sodium chloride.

• Application Notes: SDS-PAGE: 45-55kD, reducing conditions.
• Biological Activity: The specific activity is 35pmoles/min/ug.
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for REN active protein

Renin is a aspartyl protease that plays a crucial role in the regulation of blood pressure and salt balance through the rennin-angiotensin system. It cleaves angiotensinogen to generate angiotensin I, which can be fur ther converted by angiotensin converting enzyme (ACE) to angiotensin II. Angiotensin II is the active molecule of the rennin-angiotensin system that acts by binding to angiotensin receptors type 1 and 2 (AT-1 and AT-2), and has direct pathophysiological effects on the heart and peripheral vasculature. After secretion, inactive prorenin can be proteolytically activated by trypsin, cathepsin B, or other proteinases. Because of its unique specificity, i nhibition of Renin is widely studied to provide selective therapy for hypertension, congestive heart failure and associated disorders.

Product Categories/Family for REN active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4506475
• NCBI GeneID: 5972
• NCBI Accession #: NP_000528.1
• NCBI GenBank Nucleotide #: NM_000537.3
• UniProt Accession #: P00797; Q6FI38; Q6T5C2
• Molecular Weight: 43kD
• NCBI Official Full Name: renin preproprotein
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
• UniProt Protein Name: Renin
• Protein Family: Renin
• UniProt Gene Name: REN
• UniProt Entry Name: RENI_HUMAN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.23.15; Protease; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; drinking behavior; renin-angiotensin regulation of aldosterone production; male gonad development; cell maturation; response to lipopolysaccharide; proteolysis; angiotensin maturation; cellular protein metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; beta-amyloid metabolic process; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2