Platelet-derived growth factor Active Protein | PDGFB active protein

Human platelet-derived growth factor

Cat. Num. AAA430210

• Gene Names: PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• Purity: ≥ 97% purity (by SDS PAGE and HPLC)
• Synonyms: Platelet-derived growth factor; Human platelet-derived growth factor; PDGFB active protein

• Purity/Purification: ≥ 97% purity (by SDS PAGE and HPLC)
• Form/Format: Sterile lyophilized powder, pH7.4, 100 mM Glycine, 150mM NaCI buffer solution.
• Sequence Length: 241

• Source: Yeast
• Endotoxin Level: ≤ 1 EU/mg, determined by the LAL method
• Biological Activity: Measured in a cell proliferation assay using Balb/c 3T3, the specific activity shall be not less than 1 x10⁶ IU/mg
• Reconstitution: Reconstitute with double distilled water at a concentration of no less than 50 ug/ml with 0.1% human serum albumin (A highly purified plant-derived human serum albumin is strongly suggested to be used) or bovine serum albumin as a stock.
• Preparation and Storage: Stable for 6-months from the date of shipment when kept at -20°C or -70°C. Upon reconstitution, it can be stored at 4°C for at least one month or -20°C for at least three months. Avoid repeated freeze-thaw cycles.

Related Product Information for PDGFB active protein

Introduction: Platelet-derived growth factor (PDGF) is one of the numerous growth factors, or proteins that regulate cell growth and division. In particular, it plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. PDGF is a potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells.

Description: Recombinant human hPDGF produced in Yeast is a homodimer of two glycosylated, polypeptide chains containing 109 amino acid residues each, one intercellular and three intracellular pairs of disulfide bonds, having a molecular weight of 30 kDa.

Product Categories/Family for PDGFB active protein

Cytokine Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 338209
• NCBI GeneID: 5155
• NCBI Official Full Name: platelet-derived growth factor
• NCBI Official Synonym Full Names: platelet derived growth factor subunit B
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B
• UniProt Protein Name: Platelet-derived growth factor subunit B
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN

Uniprot Description

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space; Golgi membrane

Molecular Function: chemoattractant activity; collagen binding; growth factor activity; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity; superoxide-generating NADPH oxidase activator activity

Biological Process: activation of protein kinase activity; activation of protein kinase B; embryonic placenta development; extracellular matrix organization and biogenesis; heart development; hemopoiesis; MAPKKK cascade; monocyte chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of cyclin-dependent protein kinase activity; positive regulation of DNA replication; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of glomerular filtration; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of phosphoinositide 3-kinase cascade; response to wounding; transforming growth factor beta receptor signaling pathway

Disease: Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 5; Dermatofibrosarcoma Protuberans; Meningioma, Familial, Susceptibility To