NUDT5 Human, Active Protein | NUDT5 active protein

NUDT5, Recombinant Human, (ADP-sugar Pyrophosphatase, HSPC115, hYSAH1, Nucleoside Diphosphate-linked Moiety X Motif 5, Nudix Motif 5, YSA1H)

Cat. Num. AAA637861

• Gene Names: NUDT5; YSA1; YSA1H; YSAH1; hYSAH1
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: NUDT5 Human; ; NUDT5; Recombinant Human; (ADP-sugar Pyrophosphatase; HSPC115; hYSAH1; Nucleoside Diphosphate-linked Moiety X Motif 5; Nudix Motif 5; YSA1H); NUDT5 active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in Tris, sodium chloride, EDTA, DTT, Glycerol.

• Biological Activity: Measured by its ability to hydrolyze ADP-ribose to AMP and ribose-5-phosphate. The specific activty is >1,000 pmoles/min/ug, as measured under the described conditions.; ; Endotoxin Level:; <1EU/ug (LAL method)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for NUDT5 active protein

ADP­Sugar Pyrophosphatase (NUDT5)is a member of the nudi x super family of enzymes. Members of this family are pyrophosphohydrol gases that act upon substrates with the general structure of a nucleoside(Nu) diphosphate (di) linked to another moiety, X (NDP­X) to yield NMP plus P­X (1).Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP ribosyl at ion at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucl eosides (5,6).

Product Categories/Family for NUDT5 active protein

Molecular Biology; MB-Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 48146507
• NCBI GeneID: 11164
• UniProt Accession #: Q9UKK9; Q6IAG0; Q9UH49; A8K516
• Molecular Weight: ~25kD
• NCBI Official Full Name: NUDT5
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 5
• NCBI Official Symbol: NUDT5
• NCBI Official Synonym Symbols: YSA1; YSA1H; YSAH1; hYSAH1
• NCBI Protein Information: ADP-sugar pyrophosphatase; 8-oxo-dGDP phosphatase
• UniProt Protein Name: ADP-sugar pyrophosphatase
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT5
• UniProt Synonym Gene Names: Nudix motif 5
• UniProt Entry Name: NUDT5_HUMAN

NCBI Description

This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013]

Uniprot Description

NUDT5: Hydrolyzes with similar activities ADP-ribose ADP- mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. Belongs to the Nudix hydrolase family.

Protein type: EC 3.6.1.13; Nucleotide Metabolism - purine; EC 3.6.1.58; Phosphatase

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: intracellular; nucleus; cytosol

Molecular Function: snoRNA binding; m7G(5')pppN diphosphatase activity; ADP-ribose diphosphatase activity; nucleoside-diphosphatase activity; magnesium ion binding; ADP-sugar diphosphatase activity

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; D-ribose catabolic process; ribonucleoside diphosphate catabolic process