Neurexophilin 1, Rat Active Protein | NXPH1 active protein

Neurexophilin 1, Recombinant, Rat (NXPH1)

Cat. Num. AAA636137

• Gene Names: NXPH1; NPH1; Nbla00697
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Neurexophilin 1; Rat; Recombinant; Rat (NXPH1); NXPH1 active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured in a competitive binding assay. When recombinant rat neurexin-1 alpha is coated at 1ug/ml (100 L/well), rrNXPH-1 inhibits 50% binding of rrNXPH-1 biotin (1ug/ml) at the concentration range of 1-3ug/ml.
• Endotoxin Level: < 1.0 EU per 1 g of protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for NXPH1 active protein

Neurexophilin-1 (NXPH-1) is one of at least four vertebrate neuropeptide-like secreted glycoproteins in the neurexophilin family (1, 2). The 29 kD, 271 amino acid (aa) NXPH-1 sequence contains a 22 aa signal peptide, a 94 aa propeptide that is cleaved at a basic motif, and a 115 aa mature protein that contains three potential N-glycosylation sites in the N-terminal portion and six conserved cysteines in the C-terminal portion (1). Mature rat NXPH-1 shares 99%, 99%, 99%, 96% and 84% aa identity with mouse, human, bovine, opossum and zebrafish NXPH-1, respectively. NXPH-1 is expressed selectively in subpopulations of neurons within the cerebral cortex, cerebellum and olfactory bulb that are thought likely to be inhibitory interneurons (2, 3). NXPH-3 is the neurexophilin most similar to NXPH-1, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to coincide, but both are tightly bound extracellular ligands of -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 4, 5). Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show defects in motor coordination (4, 6). Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind -neurexins (1, 4).

Product Categories/Family for NXPH1 active protein

Molecular Biology; MB-Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 23097338
• NCBI GeneID: 30010
• NCBI Accession #: NP_689958.1
• NCBI GenBank Nucleotide #: NM_152745.2
• UniProt Accession #: P58417; Q8NB31
• Molecular Weight: Based on N-terminal sequencing, the recombinant rat neurexophilin-1 starts at Ala 22 and has a predicted molecular mass of 29.4 kD. A secondary sequence accounting for approximately 30% of the protein abundance begins at Arg 107. As a result of glycosylat
• NCBI Official Full Name: neurexophilin-1
• NCBI Official Synonym Full Names: neurexophilin 1
• NCBI Official Symbol: NXPH1
• NCBI Official Synonym Symbols: NPH1; Nbla00697
• NCBI Protein Information: neurexophilin-1
• UniProt Protein Name: Neurexophilin-1
• Protein Family: Neurexophilin
• UniProt Gene Name: NXPH1
• UniProt Synonym Gene Names: NPH1
• UniProt Entry Name: NXPH1_HUMAN

NCBI Description

This gene is a member of the neurexophilin family and encodes a secreted protein with a variable N-terminal domain, a highly conserved, N-glycosylated central domain, a short linker region, and a cysteine-rich C-terminal domain. This protein forms a very tight complex with alpha neurexins, a group of proteins that promote adhesion between dendrites and axons. [provided by RefSeq, Jul 2008]

Uniprot Description

NXPH1: May be signaling molecules that resemble neuropeptides and that act by binding to alpha-neurexins and possibly other receptors (Potential). Belongs to the neurexophilin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: extracellular region

Molecular Function: receptor binding