MMP-2 active protein

RECOMBINANT HUMAN MMP-2

Cat. Num. AAA232504

• Gene Names: MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
• Applications: Functional Assay
• Purity: >90% by SDS PAGE and HPLC analysis
• Synonyms: MMP-2; RECOMBINANT HUMAN MMP-2; MMP-2 active protein

• Purity/Purification: >90% by SDS PAGE and HPLC analysis
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 0.1mg/ml after reconstitution (varies by lot)
• Sequence Length: 610

• Applicable Applications for MMP-2 active protein: Functional Assays (FN)
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Preparation
• Buffer Solution: Sodium phosphate Calcium chloride
• Activity: MMP-2 activity was measured by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. At an MMP-2 concentration of 2.5 mug/ml, 50% cleavage was achieved at an incubation time of approximately 25 mins.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for MMP-2 active protein

Recombinant Human MMP-2 antigen produced in E Coli represents human MMP-2 (matrix metalloproteinase 2) containing the entire catalytic N-terminal domain and C-terminal domain. MMP-2 is otherwise known as gelatinase A, a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin. MMP-2 specifically cleaves gelatin type I, and types IV, V, VII and X collagens, and is also involved in tissue repair, inflammation, tumour invasion, vasculature angiogenesis, and the rupture of atherosclerotic plaques. Defects in the MMP2 gene are responsible for the autosomal recessive osteolysis disorders known as Torg-Winchester syndrome (Zankl et al. 2007), and multicentric osteolysis nodulosis and arthropathy (Martignetti et al. 2001).

NCBI and Uniprot Product Information

• NCBI GI #: 189217853
• NCBI GeneID: 4313
• NCBI Accession #: NP_001121363.1
• NCBI GenBank Nucleotide #: NM_001127891.2
• UniProt Accession #: P08253; Q9UCJ8; B2R6U1; B4DWH3; E9PE45
• Molecular Weight: 68,831 Da
• NCBI Official Full Name: 72 kDa type IV collagenase isoform 2
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: MMP2
• NCBI Official Synonym Symbols: CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
• NCBI Protein Information: 72 kDa type IV collagenase; collagenase type IV-A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; neutrophil gelatinase
• UniProt Protein Name: 72 kDa type IV collagenase
• UniProt Gene Name: MMP2
• UniProt Synonym Gene Names: CLG4A; MMP-2
• UniProt Entry Name: MMP2_HUMAN

NCBI Description

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.24; Secreted, signal peptide; Secreted; Apoptosis; Motility/polarity/chemotaxis; Protease; Cell development/differentiation

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; sarcomere; mitochondrion; plasma membrane; extracellular region; nucleus

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; intramembranous ossification; cellular protein metabolic process; positive regulation of innate immune response; response to hypoxia; ephrin receptor signaling pathway; angiogenesis; proteolysis; blood vessel maturation; embryo implantation

Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy