Matrix Metalloproteinase 7 Active Protein | MMP7 active protein

Matrix Metalloproteinase 7, Recombinant, Human (MMP-7, Matrilysin, Pump1, Uterine Metalloproteinase)

Cat. Num. AAA637479

• Gene Names: MMP7; MMP-7; MPSL1; PUMP-1
• Purity: Purified from human kidney cells stably transfected with full-length human MMP-7. Free of contaminating MMPs and TIMPs.
• Synonyms: Matrix Metalloproteinase 7; Recombinant; Human (MMP-7; Matrilysin; Pump1; Uterine Metalloproteinase); MMP7 active protein

• Purity/Purification: Purified from human kidney cells stably transfected with full-length human MMP-7. Free of contaminating MMPs and TIMPs.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.4, 5mM CaCl2, 0.15M sodium chloride.

• Application Notes: Suitable for use in ELISA, Western Blot (control or standard) and SDS-PAGE Standards.
• Specific Activity: ~5000 U/mg protein
• Unit Definition: One unit of enzyme activity is defined as the quantity required to digest 1ug of Azocoll/min. at pH 7.5 and 37 degree C in the presence of 0.5mM p-aminophenylmercuric acetate.
• Preparation and Storage: MMP-7, when stripped of TIMPs, is unstable, and must stored carefully. To avoid repeated freeze thaw cycles, aliquot and store the enzyme at -70 degree C. Aliquots are stable for at least 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for MMP7 active protein

H-MMP-7 is a member of the Matrix metalloproteinase family of endopeptidases, containing the canonical HExxHxxGxxH motif as a zinc binding active site. MMP-7 and MMP-26 (Matrilysin-2) are the only MMPs discovered that completely lack a hemopexin "regulatory domain" or hinge region. Like the other MMPs, MMP-7 is secreted as a zymogen, then activated. The 28kD form is reduced to 18kD by enzymatic cleavage after the conserved "cysteine switch." MMP-7 is not constitutively produced. It is produced on demand in specific tissues. MMP-7 substrate specificity is broad, most closely resembling the activity of MMP-3 (Stromelysin-1). When MMP-7 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them.

Product Categories/Family for MMP7 active protein

Molecular Biology; MB-Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 4505219
• NCBI GeneID: 4316
• NCBI Accession #: NP_002414.1
• NCBI GenBank Nucleotide #: NM_002423.3
• UniProt Accession #: P09237; Q9BTK9
• Molecular Weight: 19,130
• NCBI Official Full Name: matrilysin preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 7 (matrilysin, uterine)
• NCBI Official Symbol: MMP7
• NCBI Official Synonym Symbols: MMP-7; MPSL1; PUMP-1
• NCBI Protein Information: matrilysin; matrin; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine)
• UniProt Protein Name: Matrilysin
• Protein Family: Matrilysin
• UniProt Gene Name: MMP7
• UniProt Synonym Gene Names: MPSL1; PUMP1; MMP-7
• UniProt Entry Name: MMP7_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP7: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.23; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: response to drug; collagen catabolic process; extracellular matrix disassembly; defense response to Gram-positive bacterium; extracellular matrix organization and biogenesis; antibacterial peptide biosynthetic process; defense response to Gram-negative bacterium; proteolysis; antibacterial peptide secretion; regulation of cell proliferation