Macrophage Stimulating Protein, C672A Mutation Active Protein

Macrophage Stimulating Protein, C672A Mutation, Recombinant, Human (MSP) (BSA Free)

Cat. Num. AAA650628

• Purity: Highly Purified; 95, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Macrophage Stimulating Protein; C672A Mutation; Recombinant; Human (MSP) (BSA Free); C672A Mutation active protein

• Purity/Purification: Highly Purified; 95, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to activate MSP R/Ron in a human breast cancer cell line, MDA-MB-453. 80 ng/mL of the rhMSP (C672A) significantly induces phosphorylation of MSP R/Ron measured by DuoSet ® IC human phopho-MSP R/Ron kit.
• Endotoxin Level: < 1.0 EU per 1 g of the cytokine as determined by the LAL method. Endotoxin Level
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long termstorage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for Macrophage Stimulating Protein, C672A Mutation active protein

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45-62 kD alpha and a 25-35 kD beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP, as well as the isolated beta chain, binds to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12). The substitution of cysteine 672 (in the beta chain) with alanine significantly increases the bioactivity of recombinant MSP, apparently by limiting incorrect disulfide bond formation between the alpha and beta chains (13).

Product Categories/Family for Macrophage Stimulating Protein, C672A Mutation active protein

Growth Factors; Cytokines; Growth Factors-Other

NCBI and Uniprot Product Information

• NCBI GI #: 398038
• Molecular Weight: The mature recombinant human MSP (C672A) is a disulfide-linked heterodimer consisting of a 465 aa residue -chain (Gln 19-Arg 483) and a 228 aa residue -chain (Val 484-Gly 711) with calculated molecular masses of approximately 53 kD and 25 kD, respectively
• NCBI Official Full Name: macrophage-stimulating protein

NCBI Description

The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq, Jan 2010]

Uniprot Description

MSP: Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21

Molecular Function: serine-type endopeptidase activity

Biological Process: sperm motility; spermatogenesis; proteolysis; embryo implantation