Leptin, Rat Active Protein | Lep active protein
Leptin, Recombinant, Rat (Obese Protein, Obesity Factor)
Gene Names
Lep; OB; obese
Applications
Western Blot
Purity
Highly Purified95% (RP-HPLC, Anion exchange FPLC, SDS-PAGE).
Synonyms
Leptin; Rat; Recombinant; Rat (Obese Protein; Obesity Factor); Lep active protein
Purity/Purification
Highly Purified
95% (RP-HPLC, Anion exchange FPLC, SDS-PAGE).
95% (RP-HPLC, Anion exchange FPLC, SDS-PAGE).
Form/Format
Supplied as a lyophilized powder from 0.0045mM NaHCO3, pH 4.0. Reconstitution in sterile distilled water with 0.1% HSA or BSA for long term storage to a concentration 100ug/ml.
Applicable Applications for Lep active protein
Western Blot (WB)
Biological Activity
Determined by in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dosage of 5ug Leptin (L1670-16)/g of body weight for 7 days. Significant effects on body weight, food consumption and plasma glucose levels were observed compared to saline-treated controls.
Endotoxin Level
0.1ng/ug (1EU/ug)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O with 0.1% HSA or BSA and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Lep active protein
Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice. It seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes.
Recombinant Rat Leptin is a single, non-glycosylated, polypeptide chain containing 147 amino acid residues with a molecular weight of 16.2kD.
Recombinant Rat Leptin is a single, non-glycosylated, polypeptide chain containing 147 amino acid residues with a molecular weight of 16.2kD.
Product Categories/Family for Lep active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,866 Da
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
Lep
NCBI Official Synonym Symbols
OB; obese
NCBI Protein Information
leptin; obesity factor
UniProt Protein Name
Leptin
Protein Family
UniProt Gene Name
Lep
UniProt Synonym Gene Names
Ob
UniProt Entry Name
LEP_RAT
NCBI Description
involved in regulation of body weight, and may have a role in angiogenesis [RGD, Feb 2006]
Uniprot Description
Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location: Secreted
Probable.
Sequence similarities: Belongs to the leptin family.
Research Articles on Lep
Similar Products
Product Notes
The Lep lep (Catalog #AAA635956) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Leptin, Rat can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Lep lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leptin, Rat, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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