Interleukin 12, Rat Active Protein | Il12b active protein
Interleukin 12, Recombinant, Rat (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF)
Gene Names
Il12b; Il12
Purity
Highly Purified95% (SDS-PAGE).
Synonyms
Interleukin 12; Rat; Recombinant; Rat (IL-12; Cytotoxic Lymphocyte Maturation Factor 1; CTL Maturation Factor; TcMF; CLMF p35; Natural Killer Cell Stimulatory Factor 1; NFSK1; p35; T cell Stimulating Factor; TSF); Il12b active protein
Purity/Purification
Highly Purified
95% (SDS-PAGE).
95% (SDS-PAGE).
Form/Format
Supplied as a lyophilized powder without additives. Reconstitute with sterile dH2O to >0.1mg/ml. For long term storage it is recommended to add 0.1% HSA or BSA.
Sequence
The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
Biological Activity
ED50 range=0.01-0.05ng/ml as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated splenocytes that have been costimulated with PMA.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Il12b active protein
Interleukin 12 (IL-12) is a cytokine with important regulatory functions bridging innate and adaptive immunity. It has been proposed as an immune adjuvant for vaccination therapy of infectious diseases and malignancies. The inflammatory properties of IL-12 play an important role in the adjuvant. Interleukin 12 (IL-12) is a proinflammatory cytokine composed by two chains, p40 and p35.It plays a key role in the promotion of a Th1 immune response in the gastrointestinal mucosa. An enhanced expression of IL-12 mRNA in gastric mucosa has been reported in individuals infected by Helicobacter pylori.
Recombinant Rat IL-12 is a glycosylated disulfide linked heterodimeric polypeptide containing 503aa and having a molecular weight of 70kD comprised of disulfide-bonded 35kD (p35) and 40kD (p40) subunits.
Recombinant Rat IL-12 is a glycosylated disulfide linked heterodimeric polypeptide containing 503aa and having a molecular weight of 70kD comprised of disulfide-bonded 35kD (p35) and 40kD (p40) subunits.
Product Categories/Family for Il12b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kD
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B
NCBI Official Symbol
Il12b
NCBI Official Synonym Symbols
Il12
NCBI Protein Information
interleukin-12 subunit beta; interleukin-12 p40
Protein Family
NCBI Description
a subunit of interleukin 12 which is a cytokine that acts on T and natural killer cells [RGD, Feb 2006]
Research Articles on Il12b
Similar Products
Product Notes
The Il12b (Catalog #AAA650383) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Il e-Pro-Val at the p35 subunit and Met-Thr-Gl u-Leu-Glu at the p40 subunit. It is sometimes possible for the material contained within the vial of "Interleukin 12, Rat, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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