HIV-1 env gp120, Strain MN Active Protein
HIV-1 env gp120, Strain MN, Recombinant (Human Immunodeficiency Virus-1) (FITC)
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Gel Super Shift Assay
Purity
Purified by gel exclusion chromatography and UF concentrated.
Synonyms
HIV-1 env gp120; Strain MN; Recombinant (Human Immunodeficiency Virus-1) (FITC); Strain MN active protein
Specificity
This FITC-conjugated gp 120 binds to mouse monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.
Purity/Purification
Purified by gel exclusion chromatography and UF concentrated.
Form/Format
Supplied as a liquid in 0.1M NaPO4, 50mM Sodium Chloride, pH 7.0. No stabilizers or preservatives addded. Labeled with FITC.
Concentration
1mg/ml (varies by lot)
Applicable Applications for HIV-1 env gp120, Strain MN active protein
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Direct Immunoflouorescent staining, FACS, Immunohistochemistry and Dot Blot.
Dilution: Direct Immunoflouorescence: 1:50
Dot Blot: > =1:100 using purified anti-gp120 monoclonal or polyclonal antibodies. Optimal dilutions to be determined by the researcher.
Dilution: Direct Immunoflouorescence: 1:50
Dot Blot: > =1:100 using purified anti-gp120 monoclonal or polyclonal antibodies. Optimal dilutions to be determined by the researcher.
Source
Baculovirus derived recombinant gp 120 from HIV-1 MN strain
Biological Activity
This FITC-conjugated gp120 binds to CD4 bearing cells in FACS.
Grade
Affinity Purified
Fluorochrome/Protein Ratio
3-4
Preparation and Storage
For long term storage, aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable at -70 degree C for 6 months after receipt. Dilute required amount only prior to immediate use.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping: Ships with Dry Ice.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping: Ships with Dry Ice.
Product Categories/Family for HIV-1 env gp120, Strain MN active protein
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Product Notes
The HIV-1 env gp120, Strain MN (Catalog #AAA637783) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIV-1 env gp120, Strain MN can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA). Suitable for use in Direct Immunoflouorescent staining, FACS, Immunohistochemistry and Dot Blot. Dilution: Direct Immunoflouorescence: 1:50 Dot Blot: > =1:100 using purified anti-gp120 monoclonal or polyclonal antibodies. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HIV-1 env gp120, Strain MN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 env gp120, Strain MN, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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