Hepatocyte Growth Factor Active Protein
Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)
Purity
Highly Purified98% by by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).
Synonyms
Hepatocyte Growth Factor; Recombinant; Human (HGF; Scatter Factor; SF; Hepatopoietin A); Hepatocyte Growth Factor active protein
Purity/Purification
Highly Purified
98% by by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).
98% by by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).
Form/Format
Supplied as a lyophilized powder. Reconstitute in sterile dH2O, 0.1% HSA or BSA to a concentration > 0.1mg/ml. Can be further diluted to other aqueous solutions.
Sequence
Agrees with the seqeunce of native human HGF.
Biological Activity
Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells, was found to be 20-40ng/ml.
Dimers and Aggregates
< 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of HGF.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.
1. UV spectroscopy at 280nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 24 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Hepatocyte Growth Factor active protein
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro. It may modulate hematopoiesis.
Product Categories/Family for Hepatocyte Growth Factor active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
hepatocyte growth factor
Protein Family
Similar Products
Product Notes
The Hepatocyte Growth Factor (Catalog #AAA650745) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Agrees with the seqeunce of native human HGF. It is sometimes possible for the material contained within the vial of "Hepatocyte Growth Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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