Heparan Sulfate 2-O-sulfotransferase 1 Human, Active Protein | HS2ST1 active protein

Heparan Sulfate 2-O-sulfotransferase 1, Recombinant Human, (HS2ST1, HS2ST, 2-O-sulfotransferase, 2OST, dJ604K5.2, FLJ11317, KIAA0448, MGC131986)

Cat. Num. AAA636651

• Gene Names: HS2ST1; dJ604K5.2
• Purity: Purified; ~90% (SDS-PAGE)
• Synonyms: Heparan Sulfate 2-O-sulfotransferase 1 Human; ; Heparan Sulfate 2-O-sulfotransferase 1; Recombinant Human; (HS2ST1; HS2ST; 2-O-sulfotransferase; 2OST; dJ604K5.2; FLJ11317; KIAA0448; MGC131986); HS2ST1 active protein

• Purity/Purification: Purified; ~90% (SDS-PAGE)
• Form/Format: Supplied as a liquid in Tris and sodium chloride.

• Biological Activity: Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is > 20 pmoles/min/ug, as measured under the described conditions.
• Endotoxin Level: <1EU/ug (LAL method)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for HS2ST1 active protein

Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. Typically, it is covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and Nacetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues are either glucuronic acid or iduronic acid and maybe sulfated at the 2O position by heparan sulfate 2O sulfotransferase 1 (HS2ST1) (3, 4). HS2ST1 physically interacts in the Golgi apparatus with glucuronyl c5epimerase (5), which catalyzes the conversion of glucuronic acid to iduronic acid (6). As a consequence, 2O

Product Categories/Family for HS2ST1 active protein

Molecular Biology; MB-Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 6912420
• NCBI GeneID: 9653
• NCBI Accession #: NP_036394.1
• NCBI GenBank Nucleotide #: NM_012262.3
• UniProt Accession #: Q7LGA3; O75036; Q32NB5; Q8TAC5; Q9H441; Q9NUJ9; D3DT22
• Molecular Weight: ~36kD
• NCBI Official Full Name: heparan sulfate 2-O-sulfotransferase 1 isoform 1
• NCBI Official Synonym Full Names: heparan sulfate 2-O-sulfotransferase 1
• NCBI Official Symbol: HS2ST1
• NCBI Official Synonym Symbols: dJ604K5.2
• NCBI Protein Information: heparan sulfate 2-O-sulfotransferase 1; 2OST
• UniProt Protein Name: Heparan sulfate 2-O-sulfotransferase 1
• Protein Family: Heparan sulfate 2-O-sulfotransferase
• UniProt Gene Name: HS2ST1
• UniProt Synonym Gene Names: HS2ST; KIAA0448; 2-O-sulfotransferase; 2OST
• UniProt Entry Name: HS2ST_HUMAN

NCBI Description

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HS2ST1: Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high- affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues. Belongs to the sulfotransferase 3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.8.2.-; Motility/polarity/chemotaxis; Membrane protein, integral; Glycan Metabolism - heparan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis