Frizzled 8, Fc Chimera Active Protein
Frizzled 8, Fc Chimera, Recombinant, Mouse (FZD8)
Purity
Highly Purified95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0EU/1ug of the protein (LAL).
Synonyms
Frizzled 8; Fc Chimera; Recombinant; Mouse (FZD8); Fc Chimera active protein
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0EU/1ug of the protein (LAL).
95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0EU/1ug of the protein (LAL).
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with sterile PBS to prepare a stock solution of 250ug/ml.
Activity
Measured by its ability to interfere with the axis-inducing activity of Xwnt-8 mRNA in early Xenopus embryos. In a population of embryos, addition of 1ng of mouse Frizzled-8/Fc protein per embryo resulted in 50-100% reduction in the number of secondary axes produced by 10-20pg of Xwnt-8 mRNA.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot and store at -20 degree C/-70 degree C. Reconstituted product is stable for 3 months at --20 degree C/-70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Frizzled 8, Fc Chimera active protein
Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophilia based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Mouse Frizzled-8 (mFrz-8) was originally cloned in a PCR screen to identify additional Frizzled family members in vertebrates. There are nine murine Frizzled genes and 10 human Frizzled genes identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a seven-pass transmembrane region, and a variable-length C-terminal tail. The most conserved regions of the Frizzled proteins are the extracellular cysteine-rich domain (CRD) which spans approximately 120aa residues and contains 10 invariant cysteines, and the seven transmembrane domains. Mouse Frizzled-8 is 685 aa residues long and shows 95% and 82% amino acid identity to human and Xenopus Frizzled-8, respectively. Like other family members, Frizzled-8 has a complex expression pattern in the mouse embryo. It is expressed in the neural tube, brain, eye, somites, gut and bronchial arches. One of the best-characterized Wnt signal transduction pathways is the canonical Wnt/b-catenin pathway. It is involved in diverse biological mechanisms such as dorsal/ventral development in Xenopus embryos and mammalian tumor formation, acting via stabilization of b-catenin. Frizzled-8 is implicated in this pathway based on its ability to bind Wnt ligands that stimulate b-catenin stabilization and to influence the formation of secondary axes in Xenopus embryos. The mFrz-8 CRD has been demonstrated to bind cell surface Xenopus Wnt-8 (Xwnt-8) protein, indicating that this region is sufficient for binding ligand.
Product Categories/Family for Frizzled 8, Fc Chimera active protein
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Product Notes
The Frizzled 8, Fc Chimera (Catalog #AAA637547) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Frizzled 8, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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