Frizzled 8, Fc Chimera Active Protein
Frizzled 8, Fc Chimera, Recombinant, Human (FZD8, Frizzled-8, Fz-8, hFz8)
Purity
Highly Purified~95% as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Frizzled 8; Fc Chimera; Recombinant; Human (FZD8; Frizzled-8; Fz-8; hFz8); Fc Chimera active protein
Purity/Purification
Highly Purified
~95% as determined by SDS-PAGE and visualized by silver stain.
~95% as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.5mg/ml.
Application Notes
SDS-PAGE: 55-65kD, reducing conditions
Activity
Measured by its binding ability in a functional ELISA. In a 100ul reaction mixture containing biotinylated recombinant mouse Wnt-3a or Wnt-5a at 50-100ng/ml and recombinant human Frizzled 8/Fc Chimera dilutions at 0.1-8000ng/ml, the concentration of recombinant human Frizzled 8/Fc Chimera that produces 50% of the optimal binding response is found to be ~7-28ng/ml.l.
Endotoxin Level
1EU/1ug (LAL)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Frizzled 8, Fc Chimera active protein
Frizzled-8 is one of at least ten seven- transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor- related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes b-catenin and promotes gene transcription that is important in development and tissue maintenance. Human Frizzled-8 cDNA encodes 694aa including a 27aa signal peptide, a 247aa extracellular domain (ECD), the 7TM region (aa276-605), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa606-694). The ECD includes a cysteine-rich region (CRD, aa30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa28-172, human Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with mouse, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD. During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis. Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate b-catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein or CTGF (connective tissue growth factor) inhibits Wnt signaling. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.
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Product Notes
The Frizzled 8, Fc Chimera (Catalog #AAA637080) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 55-65kD, reducing conditions. Researchers should empirically determine the suitability of the Frizzled 8, Fc Chimera for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Frizzled 8, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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