Frizzled 10, Fc Chimera Active Protein
Frizzled 10, Fc Chimera, Recombinant, Human (FZD10)
Purity
Highly Purified90% by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Frizzled 10; Fc Chimera; Recombinant; Human (FZD10); Fc Chimera active protein
Purity/Purification
Highly Purified
90% by SDS-PAGE under reducing conditions and visualized by silver stain.
90% by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4.
Application Notes
SDS-PAGE: 56-58kD, reducing conditions
Activity
Measured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Wnt-5a immobilized on a streptavidin-coated plate at 1ug/ml can bind recombinant human Frizzled 10, Fc Chimera with an apparent Kd <35nM.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Frizzled 10, Fc Chimera active protein
Frizzled 10 is a 68kD seven pass transmembrane glycoprotein in the Frizzled family of Wnt receptors. The 205 amino acid N-terminal extracellular region of Frizzled 10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization. The C-terminal cytoplasmic tail contains a PDZ-interaction motif. PDZ motifs mediate intracellular binding to scaffolding proteins. Within the cysteine-rich domain, human Frizzled 10 shares 71% amino acid (aa) sequence identity with Frizzled 9 and 31%-46% with Frizzled 1, -2, -3, -4, -5, -6, -7 and -8. It shares 96%, 94%, 90%, and 82% aa sequence identity with chick, mouse, Xenopus and zebrafish Frizzled 10, respectively. Frizzled 10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud and airway epithelium. It is induced by Shh and co-localizes with Shh and Wnt-7a in the neural tube. In the adult, Frizzled 10 is expressed in placenta, gastric glands and colon and renal tubule epithelial cells. Frizzled 10 associates with LRP5 to transduce Wnt-7a and Wnt-7b signals, resulting in the stabilization of cytoplasmic beta-catenin. Frizzled 10 is also upregulated in some cancers and transformed cell lines. It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas.
Product Categories/Family for Frizzled 10, Fc Chimera active protein
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Product Notes
The Frizzled 10, Fc Chimera (Catalog #AAA636075) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 56-58kD, reducing conditions. Researchers should empirically determine the suitability of the Frizzled 10, Fc Chimera for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Frizzled 10, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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