FK506 Binding Protein 1A Active Protein | LOC526524 active protein
FK506 Binding Protein 1A, Recombinant, Human (FKBP1A, FK506 Binding Protein 12kD, FKBP12)
Purity
Highly Purified95%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
FK506 Binding Protein 1A; Recombinant; Human (FKBP1A; FK506 Binding Protein 12kD; FKBP12); LOC526524 active protein
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain.
95%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a liquid in 20mM HEPES, 0.1M NaCl, pH 7.5.
Activity
Measured by its ability to inhibit calcineurin phosphatase activity in the presence of FK506. The IC50 for inhibition of calcineurin activity is 28 nM. Proline isomerase activity was demonstrated but not used for quality control.
Preparation and Storage
Store frozen at -20 degree C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Related Product Information for LOC526524 active protein
FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related-compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity 2 and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).
Product Categories/Family for LOC526524 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Amino acid residues 2-108 of human FKBP12 (Accession # BC005147) with a C-terminal His6 sequence was expressed in E. coli. FKBP12 purified from the soluble fraction of disrupted E. coli has a predicted molecular weight of 12,775 daltons.
NCBI Official Full Name
FK506 binding protein 1A, 12kDa-like
NCBI Official Synonym Full Names
FK506 binding protein 1A, 12kDa-like<
NCBI Official Symbol
LOC526524
NCBI Protein Information
FK506 binding protein 1A, 12kDa-like; FK506-binding protein 1A; FK506-binding protein 1A-like
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Product Notes
The LOC526524 (Catalog #AAA637767) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FK506 Binding Protein 1A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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