FGF 8 active protein

RECOMBINANT HUMAN FGF 8

Cat. Num. AAA232308

• Gene Names: FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
• Applications: Functional Assay
• Purity: >95% by SDS PAGE and HPLC analysis
• Synonyms: FGF 8; RECOMBINANT HUMAN FGF 8; FGF 8 active protein

• Purity/Purification: >95% by SDS PAGE and HPLC analysis
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Total protein concentration 0.1mg/ml after reconstitution (varies by lot)
• Sequence Length: 215

• Applicable Applications for FGF 8 active protein: Functional Assays (FN)
• Application Notes: Functional Assays: Minimum Dilution: 0.1ng/ml; Maximum Dilution: 20ng/ml
• Reconstitution: Reconstitute with 0.25ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% Bovine Serum Albumin is recommended.; Preparation
• Activity: 2 x 106 units/mg
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for FGF 8 active protein

Recombinant human FGF8 is an E Coli-derived protein corresponding to amino acids 23-215 of human fibroblast growth factor 8. FGF8 is a secreted peptide that promotes cell growth in an autocrine manner. The peptide sequence contains an initiator methionine at the N-terminus and is homologous to the murine, canine and bovine sequences. MBS232308 has an ED50 of

NCBI and Uniprot Product Information

• NCBI GI #: 5174439
• NCBI GeneID: 2253
• NCBI Accession #: NP_006110.1
• NCBI GenBank Nucleotide #: NM_006119.4
• UniProt Accession #: P55075; Q14915; Q15766; A1A514
• Molecular Weight: Protein Molecular Weight: 22.4kD (193 amino acid sequence)
• NCBI Official Full Name: fibroblast growth factor 8 isoform B
• NCBI Official Synonym Full Names: fibroblast growth factor 8 (androgen-induced)
• NCBI Official Symbol: FGF8
• NCBI Official Synonym Symbols: HH6; AIGF; KAL6; FGF-8; HBGF-8
• NCBI Protein Information: fibroblast growth factor 8; androgen-induced growth factor; heparin-binding growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• UniProt Gene Name: FGF8
• UniProt Synonym Gene Names: AIGF; FGF-8; AIGF; HBGF-8
• UniProt Entry Name: FGF8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF8: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone- synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; type 2 fibroblast growth factor receptor binding; fibroblast growth factor receptor binding; chemoattractant activity; type 1 fibroblast growth factor receptor binding

Biological Process: gonad development; nerve growth factor receptor signaling pathway; apoptosis; cell proliferation in forebrain; adrenocorticotropin hormone secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; motor axon guidance; forebrain dorsal/ventral pattern formation; gastrulation; Wnt receptor signaling pathway through beta-catenin; mesodermal cell migration; embryonic hindlimb morphogenesis; response to organic cyclic substance; odontogenesis; BMP signaling pathway; positive chemotaxis; induction of an organ; positive regulation of cell proliferation; male genitalia development; thyroid gland development; pallium development; mesonephros development; negative regulation of neuron apoptosis; heart looping; otic vesicle formation; regulation of odontogenesis of dentine-containing teeth; negative regulation of cardiac muscle development; epidermal growth factor receptor signaling pathway; response to drug; anatomical structure morphogenesis; phosphoinositide-mediated signaling; pharyngeal system development; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; neural plate morphogenesis; MAPKKK cascade; subpallium development; forebrain morphogenesis; dorsal/ventral axon guidance; positive regulation of organ growth; patterning of blood vessels; forebrain neuron development; ureteric bud branching; midbrain-hindbrain boundary development; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; response to oxidative stress; metanephros development

Disease: Hypogonadotropic Hypogonadism 6 With Or Without Anosmia