Endostatin, Active Active Protein
Endostatin, Active, Recombinant, Mouse
Purity
Purified~95% (SDS-PAGE)
Synonyms
Endostatin; Active; Recombinant; Mouse; Active active protein
Purity/Purification
Purified
~95% (SDS-PAGE)
~95% (SDS-PAGE)
Form/Format
Supplied as a lyophillized powder from 50mM sodium phosphate, 60mM sodium chloride, 17mM citric acid, pH6.2. Reconstitute in 250ul dH2O.
Biological Activity
10ug of Endostatin injected subcutaneously once daily for 11 days resulted in 76% inhibition growth of metastases in the B16BL6 murine metastasis model.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Endostatin, Active active protein
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis.
Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. The 20kD fragment of Collagen XVIII known as Endostatin protein inhibits tumor progression and induction of endothelial cell apoptosis. Administration of Endostatin protein to mice bearing certain tumors caused tumor regression without the development of drug resistance. Endostatin protein also inhibited systemic angiogenesis, primary tumor growth, and the development of primary metastatic lesions. It also reduced the levels of antiapoptotic proteins Bcl-2 and Bcl-xL and an increase in apoptosis of endothelial cells.
Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. The 20kD fragment of Collagen XVIII known as Endostatin protein inhibits tumor progression and induction of endothelial cell apoptosis. Administration of Endostatin protein to mice bearing certain tumors caused tumor regression without the development of drug resistance. Endostatin protein also inhibited systemic angiogenesis, primary tumor growth, and the development of primary metastatic lesions. It also reduced the levels of antiapoptotic proteins Bcl-2 and Bcl-xL and an increase in apoptosis of endothelial cells.
Product Categories/Family for Endostatin, Active active protein
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Product Notes
The Endostatin, Active (Catalog #AAA637572) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Endostatin, Active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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