Desmocollin 3 Active Protein
Desmocollin 3, Recombinant, Human
Applications
Western Blot
Purity
> 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain
Synonyms
Desmocollin 3; Recombinant; Human; Desmocollin 3 active protein
Purity/Purification
> 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with 500ul of sterile PBS to 0.1mg/ml.
Concentration
~0.1mg/ml (after reconstitution) (varies by lot)
Applicable Applications for Desmocollin 3 active protein
Western Blot (WB) - Other applications not tested.
Application Notes
Western Blot: 62.9kD protein migrates as an ~ 75-85kD protein in SDS-PAGE under reducing conditions.
Optimal dilutions to be determined to the researcher.
Optimal dilutions to be determined to the researcher.
Grade
Purified
Endotoxin
<1.0 EU/ug (LAL)
N-terminal Sequence Analysis
Arg136
Activity
Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibrosblast cells. When 3 x 10e4 cells/well are added to rhDSC3 coated plates (10ug/ml, 100ul/well), approximately 45-65% will adhere after 90 minutes at 37°C.
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Desmocollin 3 active protein
Desmocollin-3 is a 100-110kD type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1-3). The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (4, 5). Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain (4, 6). Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (5, 7, 8). Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers (7). In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non-desmosomal functions during early development (9, 10). During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases (11). Desmocollin-3 can be upregulated or downregulated in carcinoma (6, 12, 13).
A DNA sequence encoding the extracellular domain of human Desmocollin-3 (DSC-3; Arg 136-Lys 690; Accession # Q14574) was fused to a 6x histidine tag at the c-terminus. The protein was expressed in a mouse myeloma cell line, NSO
A DNA sequence encoding the extracellular domain of human Desmocollin-3 (DSC-3; Arg 136-Lys 690; Accession # Q14574) was fused to a 6x histidine tag at the c-terminus. The protein was expressed in a mouse myeloma cell line, NSO
Product Categories/Family for Desmocollin 3 active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Desmocollin 3 (Catalog #AAA637447) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Desmocollin 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) - Other applications not tested. Western Blot: 62.9kD protein migrates as an ~ 75-85kD protein in SDS-PAGE under reducing conditions. Optimal dilutions to be determined to the researcher. Researchers should empirically determine the suitability of the Desmocollin 3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmocollin 3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
