DEFENSIN ALPHA 1 Active Protein | DEFA1B active protein

RECOMBINANT HUMAN DEFENSIN ALPHA 1

Cat. Num. AAA232451

• Gene Names: DEFA1B; HP1; HP-1; HNP-1
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis
• Synonyms: DEFENSIN ALPHA 1; RECOMBINANT HUMAN DEFENSIN ALPHA 1; DEFA1B active protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 0.1mg/ml after reconstitution (varies by lot)
• Sequence Length: 94

• Applicable Applications for DEFA1B active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Recombinant human defensin-alpha-1 may be used as a standard for ELISA applications with either a purified anti human defensin-alpha-1 antibody or a biotinylated anti human defensin-alpha-1 antibody.; Western Blot: Recombinant human defensin-alpha-1 may be used as a positive control for Western Blotting applications with either a purifed human defensin-alpha-1 antibody or a biotinylated human defensin-alpha-1 antibody.; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Preparation
• Activity: Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0-10.0 ng/ml.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for DEFA1B active protein

Recombinant Human Defensin-alpha-1 is a 3.4kDa peptide comprised of 30 amino acid residues corresponding to full-length mature human defenstin alpha 1. Defensin-alpha-1, otherwise known as HNP-1, is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections.

NCBI and Uniprot Product Information

• NCBI GI #: 124248516
• NCBI GeneID: 728358
• NCBI Accession #: NP_001035965.1
• NCBI GenBank Nucleotide #: NM_001042500.1
• UniProt Accession #: P59665; P11479; Q14125; Q6EZF6
• Molecular Weight: 3.4kD (30 amino acid residues)
• NCBI Official Full Name: neutrophil defensin 1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: defensin, alpha 1B
• NCBI Official Symbol: DEFA1B
• NCBI Official Synonym Symbols: HP1; HP-1; HNP-1
• NCBI Protein Information: neutrophil defensin 1
• UniProt Protein Name: Neutrophil defensin 1
• UniProt Gene Name: DEFA1
• UniProt Synonym Gene Names: DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
• UniProt Entry Name: DEF1_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to virus; defense response to fungus