Dectin 2a Active Protein

Dectin 2a, Recombinant, Mouse (CLEC6A, CLECSF10, NKCL)

Cat. Num. AAA636707

• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Dectin 2a; Recombinant; Mouse (CLEC6A; CLECSF10; NKCL); Dectin 2a active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to bind biotinylated -D-Mannose-PAA in a functional ELISA.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for Dectin 2a active protein

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% aa sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD). 1,2 Mature mouse dectin-2 consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD. 3,4 Alternate splicing leads to partial deletion of the transmembrane segment and stalk (isoform) or a portion of the CRD (isoform). 4 The full length dectin-2 isoform is a 27 kD molecule that is primarily expressed on the surface of tissue macrophages and their precursors. 3-6 The CRD of dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration. 7 It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii. 7,8 The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor subunit in macrophages. 8 Ligation of dectin-2 induces tyrosine phosphorylation of the subunit, activation of NF B, and enhanced release of TNF- and IL-1ra. 8 Macrophage dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation. 6 It mediates the breaking of UV-induced tolerance by interacting with CD4+ CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-. 9 Within the ECD, mouse dectin-2 shares 71% aa sequence identity with human and bovine dectin-2.

Product Categories/Family for Dectin 2a active protein

Molecular Biology; MB-Carbohydrates; Glycoproteins