Dectin 2 Active Protein

Dectin 2, Recombinant, Human (CLEC6A, CLECSF10, NKCL)

Cat. Num. AAA635735

• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Dectin 2; Recombinant; Human (CLEC6A; CLECSF10; NKCL); Dectin 2 active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to bind biotinylated -D-Mannose-PAA in a functional ELISA.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for Dectin 2 active protein

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% aa sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kD molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4-6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the subunit, activation of NF B, and enhanced release of TNF- and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+ CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF- (10).

Product Categories/Family for Dectin 2 active protein

Molecular Biology; MB-Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 34979184
• Molecular Weight: Based on N-terminal amino acid sequencing, the recombinant human Dectin-2 begins with the histidine tag. It has a predicted molecular mass of approximately 19.7 kD. As a result of glycosylation, the recombinant human Dectin-2 migrates as an approximately
• NCBI Official Full Name: dendritic cell-associated lectin 2

NCBI Description

Like dectin-1 (CLEC7A; MIM 606264), CLEC6A, or dectin-2, is a type II membrane receptor with an extracellular C-type lectin-like domain fold. However, unlike dectin-1, dectin-2 lacks an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain (Kanazawa et al., 2004 [PubMed 15175046]).[supplied by OMIM, Nov 2010]

Uniprot Description

dectin-2: Binds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptative immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to membrane

Molecular Function: carbohydrate binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; innate immune response; defense response to fungus; positive regulation of cytokine secretion