Creatine Kinase MB Isoenzyme Active Protein | CKM active protein

Creatine Kinase MB Isoenzyme

• Gene Names: CKM; CKMM; M-CK
• Purity: Purified
• Synonyms: Creatine Kinase MB Isoenzyme; Creatine Kinase MB (CK-MB); Human Cardiac - Lyophilized; CKM active protein

• Purity/Purification: Purified
• Form/Format: Powder lyophilized from 10 mM tris-succinate with 2 mM EDTA and 2mM DTT, pH 7.5
• Sequence Length: 381

• Source: Human Heart
• CAS Number: 9001-15-4
• UNSPSC Code: 51131907
• Unit Definition: One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37 degree C.
• Specific Activity: 84 U/mg protein
• Activity: 62 U/mg (Dimension (R) Clinical Chemistry System)
• Solubility: Clear, straw colored solution (1mg/ml saline)
• Appearance: Light yellow to tan powder
• Reconstitution: Tris buffered saline with 1% BSA, 1mM DTT and 1mM EDTA.
• Protein: 0.74 mg protein/mg (Coomassie)
• Electrophoresis: > or = 99% CKMB (Helena QuickGel ®CK); < or = 1% CKMM, CKBB
• Contaminants: GOT: 0.05%, ; LDH: 0.6% ; Ammonia: <0.1 micromole/mg solid
• Country of Origin: United States of America (Note: Donor material originates for TSE, CJD and vCJD-free counties, and mandatorily reports requirements for occurrence of these diseases.)
• Handling: Handle as a potentially hazardous substance.
• Preparation and Storage: At -20 degree C; Storage note: 2-8 Degree C.

Related Product Information for CKM active protein

MyBioSource is the leading supplier of Human Creatine Kinase MB (CK-MB) isoenzyme for manufacturing and research purposes.

Custom preparations, technical support, bulk quantities and aliquoting available.

Human Creatine Kinase is one of the most important myocardial markers (in spite of not being altogether cardiac-specific), with well-established roles in confirming acute myocardial infarction (AMI). It can also used for monitoring reperfusion during thrombolytic therapy following AMI. Testing is performed when the total Human Creatine Phosphokinase (CPK) level is elevated. Isoenzyme testing can help differentiate the source of the damaged tissue. Human CPK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type). Three different isoenzymes: Creatine Kinase MM, BB, and MB isoenzymes exist.

Product Categories/Family for CKM active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Creatine Kinase Mb (ck-mb)

NCBI and Uniprot Product Information

• NCBI GI #: 21536288
• NCBI GeneID: 1158
• NCBI Accession #: NP_001815.2
• NCBI GenBank Nucleotide #: NM_001824.4
• Molecular Weight: 84,000
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: CKMM; M-CK
• NCBI Protein Information: creatine kinase M-type; creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKM
• UniProt Synonym Gene Names: CKMM
• UniProt Entry Name: KCRM_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Uniprot Description

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation