Collectin 11 Active Protein | COLEC11 active protein

Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11)

Cat. Num. AAA638161

• Gene Names: COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
• Applications: Western Blot
• Purity: >85% as determined by SDS-PAGE under reducing conditions and visualized by silver strain.
• Synonyms: Collectin 11; Recombinant; Mouse (Collectin Kidney Protein 1; CL-K1; COLEC11); COLEC11 active protein

• Purity/Purification: >85% as determined by SDS-PAGE under reducing conditions and visualized by silver strain.
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS to a concentration of 0.1mg/ml.

• Applicable Applications for COLEC11 active protein: Western Blot (WB); Other applications not tested
• Application Notes: Dilution: Western Blot: As a result of glycosylation the recombinant mouse CL-K1 migrates as an ~33-38kD protein in SDS-PAGE under reducing conditions.; Optimal dilutions to be determined by the researcher.
• Activity: Measured by its ability to bind Alpha-L-Fucose.
• Endotoxin Level: <1EU per 1ug of protein (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for COLEC11 active protein

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1-3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37kD collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23%-32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

DNA sequence encoding mouse CL-K1 (Gln 27-Leu 272; Accession # AAI0438) was fused to a 6x polyhistidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.

Product Categories/Family for COLEC11 active protein

Molecular Biology; MB-Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13128972
• NCBI GeneID: 78989
• NCBI Accession #: NP_076932.1
• NCBI GenBank Nucleotide #: NM_024027.4
• UniProt Accession #: Q9BWP8; Q5CZ85; A1IGE4; A1IGE5; A1IGE6; A7VJJ2; A7VJJ3; A7VJJ4; A7VJJ5; B2R9M5; B4E1G0; J3KQY9
• Molecular Weight: 33-38kD
• NCBI Official Full Name: collectin-11 isoform a
• NCBI Official Synonym Full Names: collectin sub-family member 11
• NCBI Official Symbol: COLEC11
• NCBI Official Synonym Symbols: 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
• NCBI Protein Information: collectin-11; collectin kidney protein 1
• UniProt Protein Name: Collectin-11
• Protein Family: Collectin
• UniProt Gene Name: COLEC11
• UniProt Synonym Gene Names: CL-K1
• UniProt Entry Name: COL11_HUMAN

NCBI Description

This gene encodes a member of the collectin family of C-type lectins that possess collagen-like sequences and carbohydrate recognition domains. Collectins are secreted proteins that play important roles in the innate immune system by binding to carbohydrate antigens on microorganisms, facilitating their recognition and removal. The encoded protein binds to multiple sugars with a preference for fucose and mannose. Mutations in this gene are a cause of 3MC syndrome-2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

COLEC11: Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N- acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration. Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2). 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the COLEC10/COLEC11 family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: collagen; extracellular region

Molecular Function: mannose binding

Biological Process: receptor-mediated endocytosis; multicellular organismal development; developmental process

Disease: 3mc Syndrome 2