Caspase 7, active Active Protein | Casp7 active protein
Caspase 7, active, Recombinant, Human (Mch-3, ICE-LAP3, CMH-1)
Applications
Western Blot
Purity
Molecular Biology Grade90% by SDS-PAGE
Synonyms
Caspase 7; active; Recombinant; Human (Mch-3; ICE-LAP3; CMH-1); Casp7 active protein
Purity/Purification
Molecular Biology Grade
90% by SDS-PAGE
90% by SDS-PAGE
Form/Format
Supplied as a lyophilized powder. Reconstitute in sterile 15% glycerol, PBS to 1u/ul.
Applicable Applications for Casp7 active protein
Western Blot (WB)
Application Notes
Suitable for use in studying enzyme regulation, determining target substrates, screening caspase inhibitors or as a positive control in caspsae activity assays.
Specific Activity
~20,000 units/mg
Unit Definition
One unit of the recombinant caspase-7 is the enzyme activity that cleaves 1nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol, and 10mM DTT.
Preparation and Storage
Lyophilized powder may be stored at -70 degree C. Reconstitute with sterile 15% glycerol, PBS. Aliquot and store at -70 degree C. Reconstituted product is stable for 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Casp7 active protein
Caspase-7 (also know as Mch3, ICE-LAP3, CMH-1) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-7 also exists in cells as an inactive proenzyme. During apoptosis procaspase-7 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-7 consists of large and small subunits which associate to form the active enzyme. The active caspase-7 has been shown involving in the proteolysis of poly (ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance. The upstream sequence of the PARP cleavage site, DEVD, has been utilized as a basis for synthesis of caspase-3 and -7 substrates DEVD-AFC and DEVD-pNA. The active recombinant caspase-7 is routinely tested for its ability to enzymatically cleave the caspase-7 colorimetric substrate Ac-DEVD-pNA.
The active recombinant human caspase-7 was expressed in E. coli. The expressed caspase-7 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-7 preferentially cleaves the substrates consisting of consensus sequence DEVD. In combination with caspase activity assays, the active caspase-7 is useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses. We recommend using 1 unit per assay for analyzing caspase activity.
The active recombinant human caspase-7 was expressed in E. coli. The expressed caspase-7 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-7 preferentially cleaves the substrates consisting of consensus sequence DEVD. In combination with caspase activity assays, the active caspase-7 is useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses. We recommend using 1 unit per assay for analyzing caspase activity.
Product Categories/Family for Casp7 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
caspase-7
NCBI Official Synonym Full Names
caspase 7
NCBI Official Symbol
Casp7
NCBI Protein Information
caspase-7
Protein Family
NCBI Description
member of the cysteine-aspartic acid protease (caspase) family that mediates terminal phase of apoptosis [RGD, Feb 2006]
Research Articles on Casp7
Similar Products
Product Notes
The Casp7 (Catalog #AAA637680) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Caspase 7, active can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in studying enzyme regulation, determining target substrates, screening caspase inhibitors or as a positive control in caspsae activity assays. Researchers should empirically determine the suitability of the Casp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 7, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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