Caspase 6, active Active Protein | CASP10 active protein

Caspase 6, active, Recombinant, Human (Mch2)

Cat. Num. AAA636358

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Purity: Molecular Biology Grade
• Synonyms: Caspase 6; active; Recombinant; Human (Mch2); CASP10 active protein

• Purity/Purification: Molecular Biology Grade
• Form/Format: Lyophilized powder

• Specific Activity: 13,000 units/mg
• Reconstitution: Reconstitute to 1 unit per ml in PBS.
• Unit Definition: One unit of the recombinant caspase-6 is the enzyme activity that cleaves 1nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM NaCl, 0.1% Chaps, 10mM EDTA, 5% Glycerol, and 10mM DTT.
• Preparation and Storage: The lyophilized caspase-6 is stable for 1 year at -70 degree C. Following reconstitution in PBS, the enzyme should be aliquoted and stored at -70 degree C. Avoid multiple freeze/thaw cycles as activity might decrease.

Related Product Information for CASP10 active protein

Caspase-6 (also know as Mch2) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-6 consists of large (18 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-6 has been shown involving in the proteolysis of poly (ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance. The active recombinant human caspase-6 is routinely tested for its ability to enzymatically cleave the caspase-6 colorimetric substrate Ac-VEID-pNA.

The active recombinant human caspase-6 was expressed in E. coli. The expressed caspase-6 spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme. The recombinant caspase-6 cleaves the substrates consisting of consensus sequence VEID (e.g., VEID-AFC and VEID-pNA). In combination with caspase activity assays, the active recombinant caspase-6 is useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.

Product Categories/Family for CASP10 active protein

Molecular Biology; MB-Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 330864823
• NCBI GeneID: 843
• NCBI Accession #: NP_001193453.1
• NCBI GenBank Nucleotide #: NM_001206524.1
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 58,951 Da
• NCBI Official Full Name: caspase-10 isoform 6 preproprotein
• NCBI Official Synonym Full Names: caspase 10, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.63

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; plasma membrane; CD95 death-inducing signaling complex; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; innate immune response; proteolysis

Disease: Lymphoma, Non-hodgkin, Familial; Gastric Cancer; Autoimmune Lymphoproliferative Syndrome, Type Iia