Caspase 2, Active Active Protein | CASP2 active protein
Caspase 2, Active, Recombinant, Human (ICH1, Nedd-2)
Gene Names
CASP2; ICH1; CASP-2; ICH1L1S; ICH-1L/1S
Applications
Western Blot
Purity
Molecular Biology Grade
Synonyms
Caspase 2; Active; Recombinant; Human (ICH1; Nedd-2); CASP2 active protein
Purity/Purification
Molecular Biology Grade
Form/Format
Lyophilized powder
Applicable Applications for CASP2 active protein
Western Blot (WB)
Specific Activity
10,000 units/mg
Reconstitution
Reconstitute to 1 unit per ml in PBS.
Unit Definition
One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM NaCl, 0.1% Chaps, 10mM EDTA, 5% Glycerol, and 10mM DTT.
Preparation and Storage
The lyophilized caspase-2 is stable for 1 year at -70 degree C. Following reconstitution in PBS, the enzyme should be aliquoted and stored at -70 degree C. Avoid multiple freeze/thaw cycles as activity might decrease.
Related Product Information for CASP2 active protein
Recombinant Human Caspases
MyBioSource offers the most comprehensive list of highly active caspases. The recombinant forms of active caspases were expressed in E. coli and puri ed, most with purity >90% by SDS-PAGE. Active caspases are very useful in studying enzyme regulation and kinetics, screening caspase inhibitors, determining target substrates, and as positive controls in caspase activity assays and Western blot analyses, etc. One unit of each active enzyme is suf cient to cleave 1 nmol of the corresponding caspase substrate per hour at 37 degree C. Procaspase-3 are also available for studying caspase activation pathways and mechanisms.
Caspase-2 (also know as Ich-1, Nedd-2) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kD) and small (12kD) subunits which associate to form the active enzyme. The active recombinant human caspase-2 was expressed in E. coli. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA. The signal can be detected by spectrophotometry.
MyBioSource offers the most comprehensive list of highly active caspases. The recombinant forms of active caspases were expressed in E. coli and puri ed, most with purity >90% by SDS-PAGE. Active caspases are very useful in studying enzyme regulation and kinetics, screening caspase inhibitors, determining target substrates, and as positive controls in caspase activity assays and Western blot analyses, etc. One unit of each active enzyme is suf cient to cleave 1 nmol of the corresponding caspase substrate per hour at 37 degree C. Procaspase-3 are also available for studying caspase activation pathways and mechanisms.
Caspase-2 (also know as Ich-1, Nedd-2) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kD) and small (12kD) subunits which associate to form the active enzyme. The active recombinant human caspase-2 was expressed in E. coli. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA. The signal can be detected by spectrophotometry.
Product Categories/Family for CASP2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,960 Da
NCBI Official Full Name
caspase-2
NCBI Official Synonym Full Names
caspase 2, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP2
NCBI Official Synonym Symbols
ICH1; CASP-2; ICH1L1S; ICH-1L/1S
NCBI Protein Information
caspase-2; ICH1 protease; protease ICH-1; cysteine protease caspase-2; caspase 2, apoptosis-related cysteine protease; caspase 2, apoptosis-related cysteine peptidase (neural precursor cell expressed, developmentally down-regulated 2)
UniProt Protein Name
Caspase-2
Protein Family
UniProt Gene Name
CASP2
UniProt Synonym Gene Names
ICH1; CASP-2
UniProt Entry Name
CASP2_CHICK
Uniprot Description
Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival
By similarity.
Catalytic activity: Strict requirement for an Asp residue at P1, with 316-asp being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-|-.
Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit
By similarity.
Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.
Sequence caution: The sequence AAC29881.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on CASP2
Similar Products
Product Notes
The CASP2 casp2 (Catalog #AAA636734) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Caspase 2, Active can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CASP2 casp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 2, Active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
